Molecular Human Reproduction, Vol 4, 957-965, Copyright © 1998 by Oxford University Press
J Eckert and H Niemann
Leukaemia inhibitory factor (LIF) plays an essential role during cell
differentiation and implantation in the mouse exerting its effects via the
two dimerizing receptor subunits glycoprotein 130 (gp130) and LIF- receptor
beta (LR-beta). This study investigated the mRNA expression of bovine LIF
(bLIF), gp130 and LR-beta in pooled or individual bovine oocytes and
embryos generated in vitro or in vivo after superovulation. Transcripts of
gp130 and LR-beta were detectable in as little material as 1/3 oocyte
equivalent and bLIF transcripts in two embryo equivalents employing a
simple, rapid and robust one-step reverse transcription- polymerase chain
reaction (RT-PCR) protocol using random hexamer priming during reverse
transcription and 60 amplification cycles in the PCR. All mRNA fragments
were also found in cumulus cells and various bovine tissues. While gp130
and LR-beta mRNA were present in pooled material throughout embryo
development in vitro from the immature oocyte to the hatched blastocyst,
bLIF-transcripts were absent in immature oocytes, inconsistently expressed
from the matured oocyte up to the 16-cell stage as well as in blastocysts
dependent on embryo batch. It was not found in morulae, but again present
in hatched blastocysts. In contrast, in in-vivo derived embryos no bLIF was
found, LR-beta was not detected at the morula to blastocyst transition
while gp130 transcripts were observed from the morula to the hatched
blastocyst. In individual embryos the mRNA expression pattern was similar
for both in-vitro and in-vivo derived embryos as found in pooled material.
These results indicate perturbation of the mRNA expression pattern of the
specific LIF-LIF-receptor system in embryos generated in vitro that could
lead to abnormal differentiation of the cell compartments forming the
blastocyst. Cumulus cells, frequently used as supportive factor in
co-culture in bovine in-vitro embryo production, are a rich source of the
LIF-LIF-receptor system acting in a paracrine and/or autocrine manner. The
biological function of the LIF- LIF-receptor system during bovine
preimplantation development warrants further investigation.
JOURNAL ARTICLE
mRNA expression of leukaemia inhibitory factor (LIF) and its receptor subunits glycoprotein 130 and LIF-receptor-beta in bovine embryos derived in vitro or in vivo
Department of Biotechnology, Institut fur Tierzucht und Tierverhalten (FAL) Mariensee, Neustadt, Germany.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  F. C. Thomas, B. Sheth, J. J. Eckert, G. Bazzoni, E. Dejana, and T. P. Fleming Contribution of JAM-1 to epithelial differentiation and tight-junction biogenesis in the mouse preimplantation embryo J. Cell Sci., November 1, 2004; 117(23): 5599 - 5608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Wrenzycki, D. Herrmann, and H. Niemann Timing of Blastocyst Expansion Affects Spatial Messenger RNA Expression Patterns of Genes in Bovine Blastocysts Produced In Vitro Biol Reprod, June 1, 2003; 68(6): 2073 - 2080. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Ghassemifar, J. J. Eckert, F. D. Houghton, H. M. Picton, H. J. Leese, and T. P. Fleming Gene expression regulating epithelial intercellular junction biogenesis during human blastocyst development in vitro Mol. Hum. Reprod., May 1, 2003; 9(5): 245 - 252. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Miller, J. J. Eckert, G. Lazzari, V. Duranthon-Richoux, J. Sreenan, D. Morris, C. Galli, J.-P. Renard, and T. P. Fleming Tight Junction Messenger RNA Expression Levels in Bovine Embryos are Dependent upon the Ability to Compact and In Vitro Culture Methods Biol Reprod, April 1, 2003; 68(4): 1394 - 1402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rizos, A. Gutierrez-Adan, S. Perez-Garnelo, J. de la Fuente, M.P. Boland, and P. Lonergan Bovine Embryo Culture in the Presence or Absence of Serum: Implications for Blastocyst Development, Cryotolerance, and Messenger RNA Expression Biol Reprod, January 1, 2003; 68(1): 236 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rizos, P. Lonergan, M.P. Boland, R. Arroyo-Garcia, B. Pintado, J. d. l. Fuente, and A. Gutierrez-Adan Analysis of Differential Messenger RNA Expression Between Bovine Blastocysts Produced in Different Culture Systems: Implications for Blastocyst Quality Biol Reprod, March 1, 2002; 66(3): 589 - 595. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. R. Ghassemifar, B. Sheth, T. Papenbrock, H. J. Leese, F. D. Houghton, and T. P. Fleming Occludin TM4-: an isoform of the tight junction protein present in primates lacking the fourth transmembrane domain J. Cell Sci., January 8, 2002; 115(15): 3171 - 3180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Wrenzycki, D. Herrmann, L. Keskintepe, A. Martins Jr, S. Sirisathien, B. Brackett, and H. Niemann Effects of culture system and protein supplementation on mRNA expression in pre-implantation bovine embryos Hum. Reprod., May 1, 2001; 16(5): 893 - 901. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Laurincik, P.D. Thomsen, A. Hay-Schmidt, B. Avery, T. Greve, R.L. Ochs, and P. Hyttel Nucleolar Proteins and Nuclear Ultrastructure in Preimplantation Bovine Embryos Produced In Vitro Biol Reprod, April 1, 2000; 62(4): 1024 - 1032. [Abstract] [Full Text]
|
|
|
|
|
|
K. C. Reinhart, R. K. Dubey, P. J. Keller, U. Lauper, and M. Rosselli Xeno-oestrogens and phyto-oestrogens induce the synthesis of leukaemia inhibitory factor by human and bovine oviduct cells Mol. Hum. Reprod., October 1, 1999; 5(10): 899 - 907. [Abstract] [Full Text] [PDF]
|
|