Molecular Human Reproduction, Vol 4, 972-977, Copyright © 1998 by Oxford University Press
J Liu, YL Tsai, XZ Zheng, RA Yazigi, TA Baramki, G Compton and E Katz
In order to increase the number of chromosomes examined in each blastomere,
we have developed a repeated fluorescent in-situ hybridization (FISH)
procedure by which six or more chromosomes can be analysed per blastomere
of a human embryo. Three consecutive FISH procedures with directly-labelled
fluorescent Vysis DNA probes were carried out for examination of
chromosomes X, Y, 11, 13, 18 and 21 in the same blastomeres (n = 126) and
lymphocytes (n = 164). Based on the initial number of nuclei, the
percentages of nuclear loss and presence of signals were 3 and 92%
respectively in blastomeres; 6 and 91% respectively in lymphocytes after
the first FISH; 7 and 87% respectively in blastomeres and 10 and 86%
respectively in lymphocytes, after the second FISH. These percentages were
13 and 78% respectively in blastomeres and 14 and 81% respectively in
lymphocytes after the third FISH. The FISH procedure was repeated
successfully in a couple for preimplantation genetic diagnosis of
chromosomal aneuploidies in biopsied blastomeres of their embryos in our
clinic. In conclusion, it is feasible to carry out repeated FISH procedures
in the same blastomeres. Six or more chromosomes of a single blastomere may
be examined using this procedure.
JOURNAL ARTICLE
Feasibility study of repeated fluorescent in-situ hybridization in the same human blastomeres for preimplantation genetic diagnosis
The Greater Baltimore Medical Centre, Fertility Centre, MD 21204, USA.
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