Molecular Human Reproduction, Vol 4, 985-989, Copyright © 1998 by Oxford University Press
Y Yokoyama, Y Takahashi, A Shinohara, Z Lian, W Xiaoyun, K Niwa and T Tamaya
Telomerase activity is associated with the proliferative activity of cells.
In the endometrium, telomerase activity is higher in the proliferative
phase than in the secretory phase of the menstrual cycle, suggesting that
telomerase activity may occur primarily in the glandular epithelial cells.
To test this, a dissociated cell culture of the endometrium was performed,
and the telomerase activity in each cell fraction was analysed. Telomerase
activity was found in all 10 endometrial tissues of the proliferative phase
of the menstrual cycle. Both the fragments of epithelial glands and single
cells, which were prepared by enzymatic dissociation, showed telomerase
activity. In the 7 day cell culture, it was found in nine out of 10
epithelial cell enriched fractions, but in none of the stromal cell
enriched fractions. Flow cytometric analysis showed that the epithelial
enriched fraction was contaminated with a predominant number of stromal
cells, while the stromal cell enriched fraction was comprised mostly of
stromal cells with apparent proliferative activity. Our results suggest
that telomerase activity of the endometrium occurs primarily in the
epithelial cells in the endometrium and that the stromal cells do not
express telomerase activity regardless of their potent proliferative
activity.
JOURNAL ARTICLE
Telomerase activity is found in the epithelial cells but not in the stromal cells in human endometrial cell culture
Department of Obstetrics and Gynecology, Gifu University School of Medicine, Gifu, Japan.
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