Molecular Human Reproduction, Vol 4, 990-998, Copyright © 1998 by Oxford University Press
T Kamijo, MR Rajabi, H Mizunuma and Y Ibuki
During embryo implantation, apoptosis is observed morphologically at the
implantation site of endometrium. The objectives of this study were to
demonstrate biochemical evidence of apoptosis and quantitative assessment
of DNA fragmentation in uterine epithelial cells using a mouse implantation
model, and to investigate the autocrine/paracrine regulation of apoptosis
in uterine epithelial cells during blastocyst outgrowth. Blastocysts from
day 4 pregnant mice were cultured on uterine epithelial cells for 96 h.
Uterine epithelial cells dislodged by trophoblasts in
endometrium-trophoblast unit demonstrated morphological features of
apoptosis by Acridine Orange staining. Electrophoresis demonstrated DNA
ladder and DNA fragmentation by enzyme- linked immunosorbent assay markedly
increased after 48 h period of incubation. Apoptosis increased in an
exponential way in accordance with trophoblast outgrowth. In addition, DNA
fragmentation was shown in the epithelial cells by adding
embryo-conditioned medium (CM) and the effect of embryo CM on apoptosis was
significantly inhibited by anti- transforming growth factor (TGF)-beta
antibody. Delayed outgrowth was observed after 48 h of incubation in the
blastocysts cultured with anti- TGF-beta antibody. These results suggest
there is autocrine/paracrine regulation of apoptosis in uterine epithelial
cells at mouse embryo implantation and that TGF-beta might play an
important role in the occurrence of apoptosis in the
endometrium-trophoblast unit.
JOURNAL ARTICLE
Biochemical evidence for autocrine/paracrine regulation of apoptosis in cultured uterine epithelial cells during mouse embryo implantation in vitro
Department of Obstetrics and Gynecology, University of Medicine and Dentistry of New Jersey, Newark 07103, USA.
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