Molecular Human Reproduction, Vol 4, 203-206, Copyright © 1998 by Oxford University Press
J Neulen, S Raczek, M Pogorzelski, K Grunwald, TK Yeo, HF Dvorak, HA Weich and M Breckwoldt
Vascularization is a prominent event during corpus luteum formation,
providing low density lipoproteins for steroid biosynthesis and enabling
transport of secreted steroids. The process of vascularization is
controlled by specific regulators. Vascular endothelial growth factor
(VEGF), otherwise named vascular permeability factor (VPF), induces
endothelial cell proliferation as well as angiogenesis in vivo and
increases capillary permeability. Here we report the expression of VEGF/VPF
mRNA by cultured human luteinized granulosa cells (GC) for at least 10
days. Without HCG VEGF/VPF expression declined after day 4 and by day 10
was reduced to approximately 30% of the value at day 4. However, after
culture in the presence of 1 U/ml human chorionic gonadotrophin (HCG),
expression of VEGF/VPF mRNA by GC was four times greater than control
experiments by day 10, and increased 100% from day 4 to day 10.
Simultaneously, HCG supplementation increased VEGF/VPF secretion by GC.
Medium VEGF/VPF on day 3 was 13 pM without and 11 pM with HCG. Medium
VEGF/VPF on day 10 was 6 pM without HCG and 29 pM with HCG. These results
suggest that vascularization of the corpus luteum is induced by
HCG-mediated effects of VEGF/VPF.
JOURNAL ARTICLE
Secretion of vascular endothelial growth factor/vascular permeability factor from human luteinized granulosa cells is human chorionic gonadotrophin dependent
Department of Gynaecology, Endocrinology and Reproductive Medicine, University Clinic RWTH, Aachen, Germany.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. van den Driesche, M. Myers, E. Gay, K. J. Thong, and W. C. Duncan HCG up-regulates hypoxia inducible factor-1 alpha in luteinized granulosa cells: implications for the hormonal regulation of vascular endothelial growth factor A in the human corpus luteum Mol. Hum. Reprod., August 1, 2008; 14(8): 455 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Manau, F. Fabregues, J. Penarrubia, M. Creus, F. Carmona, G. Casals, W. Jimenez, and J. Balasch Vascular endothelial growth factor levels in serum and plasma from patients undergoing controlled ovarian hyperstimulation for IVF Hum. Reprod., March 1, 2007; 22(3): 669 - 675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Berndt, S. P. d'Hauterive, S. Blacher, C. Pequeux, S. Lorquet, C. Munaut, M. Applanat, M. A. Herve, N. Lamande, P. Corvol, et al. Angiogenic activity of human chorionic gonadotropin through LH receptor activation on endothelial and epithelial cells of the endometrium FASEB J, December 1, 2006; 20(14): 2630 - 2632. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. De, J.-I. Park, K. Kawamura, R. Chen, C. Klein, R. Rauch, S. M. Mulders, M. D. Sollewijn Gelpke, and A. J. W. Hsueh Intraovarian Tumor Necrosis Factor-Related Weak Inducer of Apoptosis/Fibroblast Growth Factor-Inducible-14 Ligand-Receptor System Limits Ovarian Preovulatory Follicles from Excessive Luteinization Mol. Endocrinol., October 1, 2006; 20(10): 2528 - 2538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R Gruemmer, L Klein-Hitpass, and J Neulen Regulation of gene expression in endothelial cells: the role of human follicular fluid J. Mol. Endocrinol., February 1, 2005; 34(1): 37 - 46. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Schwarzenbach, G. Chakrabarti, H. J. Paust, and A. K. Mukhopadhyay Gonadotropin-Mediated Regulation of the Murine VEGF Expression in MA-10 Leydig Cells J Androl, January 1, 2004; 25(1): 128 - 139. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Bartha, R. Romero-Carmona, M. Escobar-Llompart, O. Paloma-Castro, and R. Comino-Delgado Human Chorionic Gonadotropin and Vascular Endothelial Growth Factor in Normal and Complicated Pregnancies Obstet. Gynecol., November 1, 2003; 102(5): 995 - 999. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kisliouk, N. Levy, A. Hurwitz, and R. Meidan Presence and Regulation of Endocrine Gland Vascular Endothelial Growth Factor/Prokineticin-1 and Its Receptors in Ovarian Cells J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3700 - 3707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. SASSON, A. DANTES, K. TAJIMA, and A. AMSTERDAM Novel genes modulated by FSH in normal and immortalized FSH-responsive cells: new insights into the mechanism of FSH action FASEB J, July 1, 2003; 17(10): 1256 - 1266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Rowe, K. D. Morris, R. Bicknell, and H. M. Fraser Angiogenesis in the Corpus Luteum of Early Pregnancy in the Marmoset and the Effects of Vascular Endothelial Growth Factor Immunoneutralization on Establishment of Pregnancy Biol Reprod, October 1, 2002; 67(4): 1180 - 1188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. C. Zimmermann, E. Xiao, P. Bohlen, and M. Ferin Administration of Antivascular Endothelial Growth Factor Receptor 2 Antibody in the Early Follicular Phase Delays Follicular Selection and Development in the Rhesus Monkey Endocrinology, July 1, 2002; 143(7): 2496 - 2502. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. M. Moulton, P. H. Yoshihara, D. H. Mason, P. L. Iversen, and P. L. Triozzi Active Specific Immunotherapy with a {beta}-Human Chorionic Gonadotropin Peptide Vaccine in Patients with Metastatic Colorectal Cancer: Antibody Response Is Associated with Improved Survival Clin. Cancer Res., July 1, 2002; 8(7): 2044 - 2051. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Quintana, L. Kopcow, G. Marconi, C. Sueldo, G. Speranza, and R.I. Baranao Relationship of ovarian stimulation response with vascular endothelial growth factor and degree of granulosa cell apoptosis Hum. Reprod., September 1, 2001; 16(9): 1814 - 1818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Neulen, D. Wenzel, C. Hornig, E. Wunsch, U. Weissenborn, K. Grunwald, R. Buttner, and H. Weich Poor responder-high responder: the importance of soluble vascular endothelial growth factor receptor 1 in ovarian stimulation protocols Hum. Reprod., April 1, 2001; 16(4): 621 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wulff, H. Wilson, P. Largue, W. C. Duncan, D. G. Armstrong, and H. M. Fraser Angiogenesis in the Human Corpus Luteum: Localization and Changes in Angiopoietins, Tie-2, and Vascular Endothelial Growth Factor Messenger Ribonucleic Acid J. Clin. Endocrinol. Metab., November 1, 2000; 85(11): 4302 - 4309. [Abstract] [Full Text]
|
|
|
|
|
|
S. E. Dickson and H. M. Fraser Inhibition of Early Luteal Angiogenesis by Gonadotropin-Releasing Hormone Antagonist Treatment in the Primate J. Clin. Endocrinol. Metab., June 1, 2000; 85(6): 2339 - 2344. [Abstract] [Full Text]
|
|
|
|
|
|
N. Otani, S. Minami, M. Yamoto, T. Shikone, H. Otani, R. Nishiyama, T. Otani, and R. Nakano The Vascular Endothelial Growth Factor/fms-Like Tyrosine Kinase System in Human Ovary during the Menstrual Cycle and Early Pregnancy J. Clin. Endocrinol. Metab., October 1, 1999; 84(10): 3845 - 3851. [Abstract] [Full Text] [PDF]
|
|