Molecular Human Reproduction, Vol 4, 207-214, Copyright © 1998 by Oxford University Press
SK Roy and AR Kole
The expression patterns of transforming growth factor (TGF)-beta receptor
type I (TbetaRI) and -II (TbetaRII) in pre- and post- menopausal human
ovaries, and the in-vitro effects of follicle stimulating hormone (FSH),
epidermal growth factor (EGF) and transforming growth factor-beta1
(TGF-beta1) on receptor expression in preantral follicles were evaluated
using immunohistochemistry and immunoblotting. Both types of receptor were
present in granulosa, theca and interstitial cells; however, more mural
than antral granulosa cells were TbetaRII positive. Overall, more cells
expressed TbetaRI than TbetaRII. TbetaRI and TbetaRII expressions were
detected in the membrane as well as in the soluble fractions. However,
marked increases in TbetaRII and TbetaRI expression in the soluble fraction
and corresponding decreases in the membrane fraction were noted in the
post- menopausal ovary. Preantral follicles in class 1 and 2 responded in
vitro to FSH and EGF with increased growth and a corresponding increase in
TbetaRII expression in granulosa cells; however, TGF-beta did not influence
follicular growth or receptor expression. There was no apparent change in
follicular TbetaRI immunoreactivity regardless of test factors or
follicular growth status. These results suggest that TbetaRI and TbetaRII
are expressed differentially in human ovarian cells, particularly in the
granulosa cells. The shift in TbetaR-I and - II from transmembrane to
soluble fraction after menopause indicates that the endocrine milieu
provided by the granulosa cells positively influences receptor induction in
the membrane and, hence, the biological action of TGF-beta. FSH- and
EGF-induced preantral follicular development is associated with a selective
induction of TbetaRII and may indicate a mechanism whereby gonadotrophins
and growth factor(s) regulate preantral folliculogenesis.
JOURNAL ARTICLE
Ovarian transforming growth factor-beta (TGF-beta) receptors: in-vitro effects of follicle stimulating hormone, epidermal growth factor and TGF-beta on receptor expression in human preantral follicles
Leland J. and Dorothy H. Olson Center for Women's Health, Department of OB/GYN, University of Nebraska Medical Center, Omaha 68198-4515, USA.
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