Molecular Human Reproduction, Vol 4, 227-233, Copyright © 1998 by Oxford University Press
K Steger, I Aleithe, H Behre and M Bergmann
The quantitative distribution pattern of Ki-67 protein and proliferating
cell nuclear antigen (PCNA) immunoreactivity was studied in human testis
biopsies. In normal seminiferous epithelium Ki-67 is expressed in nuclei of
spermatogonia, while PCNA additionally occurs in nuclei of primary
spermatocytes. The staining pattern of spermatogonia is as follows
(Ki-67-positive/PCNA-positive): 26.6 +/- 12.4%/46.3 +/- 9.5%. No
stage-dependent differences were found. Biopsies with mixed atrophy (score
< or =7) showed a significant (P < 0.05) decrease of immunopositive
spermatogonia to 19.9 +/- 3.0%/31.4 +/- 5.7% (score 1) with minimal
variation between different samples (score 7 to 1). Associated with defined
histological defects such as hypospermatogenesis (hyp), spermatogenic
arrest at the level of spermatids (sda), spermatocytes (sca) or
spermatogonia (sga), however, there was a significant (P < 0.05)
decrease of Ki-67 staining in tubules showing hyp (28.6 +/- 8.8%), sda
(25.6 +/- 9.3%), sca (23.7 +/- 9.3%) and sga (16.2 +/- 6.0%) and of PCNA
staining in sca (32.2 +/- 11.8%) and sga (20.0 +/- 9.5%), respectively. The
decrease of immunoreactive spermatogonia did not correspond to elevation of
follicle stimulating hormone (FSH). These data demonstrate that the low
spermatogenic efficiency in infertile men is not only due to postmeiotic
events, but also to a decrease in the meiotic activity of spermatogonia,
and is not related to serum FSH.
JOURNAL ARTICLE
The proliferation of spermatogonia in normal and pathological human seminiferous epithelium: an immunohistochemical study using monoclonal antibodies against Ki-67 protein and proliferating cell nuclear antigen
Institute of Anatomy and Cell Biology, University of Halle, Halle (Saale), Germany.
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