Molecular Human Reproduction, Vol 4, 269-280, Copyright © 1998 by Oxford University Press
J Cohen, R Scott, M Alikani, T Schimmel, S Munne, J Levron, L Wu, C Brenner, C Warner and S Willadsen
Ooplasmic transplantation aimed at restoring normal growth in
developmentally compromised oocytes and embryos was evaluated in seven
couples (eight cycles) with multiple implantation failures. Two approaches
were investigated to transfer ooplasm from donor eggs at metaphase II (MII)
stage into patient MII eggs: (i) electrofusion of a ooplasmic donor
fragment into each patient egg (three cycles), and (ii) direct injection of
a small amount of ooplasm from a donor egg into each patient egg (five
cycles). Some donor eggs were used multiple times. Donor eggs were divided
into two groups, one being used for ooplasmic extraction and the other one
for egg donation. Cleaved embryos resulting from the latter were
cryopreserved, where numbers and satisfactory development permitted. A
second control group consisted of embryos derived from patient eggs after
intracytoplasmic sperm injection without ooplasmic transfer. This was
performed when sufficient number of eggs were available (n = 5). Donor eggs
(n = 40) were evaluated cytogenetically after micromanipulation in order to
confirm the presence of chromosomes. One egg was anuclear and the recipient
embryos were not transferred. Normal fertilization was significantly higher
after injection of ooplasm (63%) in comparison with fusion (23%).
Pronuclear anomalies appeared enhanced after fusion with ooplasts. Embryo
morphology was not improved in the three cycles with electrofusion and
patients did not become pregnant. An improvement in embryo morphology was
noted in two patients after injection of ooplasm and both became pregnant,
but one miscarried. A third pregnancy was established in the repeat
patient, without obvious embryo improvement. One baby was born and the
third pregnancy is ongoing with a normal karyotype. Two other patients with
male factor infertility had poor embryos after ooplasmic injection, but the
donor embryo controls were also poor. The patients did not become pregnant
and had no donor embryos frozen. Ooplasmic transfer at the MII stage may be
promising in patients with compromised embryos; however, evaluation of
ooplasmic anomalies and optimization of techniques will require further
investigation prior to widescale application.
JOURNAL ARTICLE
Ooplasmic transfer in mature human oocytes
The Institute for Reproductive Medicine and Science of Saint Barnabas, Livingston, New Jersey, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y.-L. Miao, K. Kikuchi, Q.-Y. Sun, and H. Schatten Oocyte aging: cellular and molecular changes, developmental potential and reversal possibility Hum. Reprod. Update, May 8, 2009; (2009) dmp014v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Cheng, K. Wang, L. D. Kellam, Y. S. Lee, C.-G. Liang, Z. Han, N. R. Mtango, and K. E. Latham Effects of Ooplasm Manipulation on DNA Methylation and Growth of Progeny in Mice Biol Reprod, March 1, 2009; 80(3): 464 - 472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.L. Bredenoord, G. Pennings, and G. de Wert Ooplasmic and nuclear transfer to prevent mitochondrial DNA disorders: conceptual and normative issues Hum. Reprod. Update, November 1, 2008; 14(6): 669 - 678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hamatani, M. Yamada, H. Akutsu, N. Kuji, Y. Mochimaru, M. Takano, M. Toyoda, K. Miyado, A. Umezawa, and Y. Yoshimura What can we learn from gene expression profiling of mouse oocytes? Reproduction, May 1, 2008; 135(5): 581 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.M Acton, I Lai, X Shang, A Jurisicova, and R.F Casper Neutral Mitochondrial Heteroplasmy Alters Physiological Function in Mice Biol Reprod, September 1, 2007; 77(3): 569 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Vandaele, B. Mateusen, D. G D Maes, A. de Kruif, and A. Van Soom Temporal detection of caspase-3 and -7 in bovine in vitro produced embryos of different developmental capacity Reproduction, April 1, 2007; 133(4): 709 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.J. Margalioth, A. Ben-Chetrit, M. Gal, and T. Eldar-Geva Investigation and treatment of repeated implantation failure following IVF-ET Hum. Reprod., December 1, 2006; 21(12): 3036 - 3043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.J.A.M. Jacobs, G. de Wert, J.P.M. Geraedts, I.F.M. de Coo, and H.J.M. Smeets The transmission of OXPHOS disease and methods to prevent this Hum. Reprod. Update, March 1, 2006; 12(2): 119 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H El Shourbagy, E. C Spikings, M. Freitas, and J. C St John Mitochondria directly influence fertilisation outcome in the pig Reproduction, February 1, 2006; 131(2): 233 - 245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Takeda, M. Tasai, M. Iwamoto, A. Onishi, T. Tagami, K. Nirasawa, H. Hanada, and C. A. Pinkert Microinjection of Cytoplasm or Mitochondria Derived from Somatic Cells Affects Parthenogenetic Development of Murine Oocytes Biol Reprod, June 1, 2005; 72(6): 1397 - 1404. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Roth and P.J. Hansen Involvement of Apoptosis in Disruption of Developmental Competence of Bovine Oocytes by Heat Shock During Maturation Biol Reprod, December 1, 2004; 71(6): 1898 - 1906. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hamatani, G. Falco, M. G. Carter, H. Akutsu, C. A. Stagg, A. A. Sharov, D. B. Dudekula, V. VanBuren, and M. S.H. Ko Age-associated alteration of gene expression patterns in mouse oocytes Hum. Mol. Genet., October 1, 2004; 13(19): 2263 - 2278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ebner, M. Moser, M. Sommergruber, K. Jesacher, and G. Tews Complete oocyte activation failure after ICSI can be overcome by a modified injection technique Hum. Reprod., August 1, 2004; 19(8): 1837 - 1841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C St John, R. E I Lloyd, E. J Bowles, E. C Thomas, and S. El Shourbagy The consequences of nuclear transfer for mammalian foetal development and offspring survival. A mitochondrial DNA perspective Reproduction, June 1, 2004; 127(6): 631 - 641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Levy, K. Elder, and Y. Menezo Cytoplasmic transfer in oocytes: biochemical aspects Hum. Reprod. Update, May 1, 2004; 10(3): 241 - 250. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B.M. Acton, A. Jurisicova, I. Jurisica, and R.F. Casper Alterations in mitochondrial membrane potential during preimplantation stages of mouse and human embryo development Mol. Hum. Reprod., January 1, 2004; 10(1): 23 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Liu, H. C. Chang, J. Zhang, J. Grifo, and L. C. Krey Metaphase II nuclei generated by germinal vesicle transfer in mouse oocytes support embryonic development to term Hum. Reprod., September 1, 2003; 18(9): 1903 - 1907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Ricken and C. Viebahn Stage-Specific Expression of the Mitochondrial Germ Cell Epitope PG2 During Postnatal Differentiation of Rabbit Germ Cells Biol Reprod, July 1, 2002; 67(1): 196 - 203. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Hawes, C. Sapienza, and K. E. Latham Ooplasmic donation in humans: The potential for epigenic modifications Hum. Reprod., April 1, 2002; 17(4): 850 - 852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Templeton Ooplasmic Transfer -- Proceed with Care N. Engl. J. Med., March 7, 2002; 346(10): 773 - 775. [Full Text] [PDF]
|
|
|
|
|
|
M.-L. Windt, K. Coetzee, T.F. Kruger, H. Marino, M.S. Kitshoff, and M. Sousa Ultrastructural evaluation of recurrent and in-vitro maturation resistant metaphase I arrested oocytes: Case report Hum. Reprod., November 1, 2001; 16(11): 2394 - 2398. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Dale, M. Wilding, G. Botta, M. Rasile, M. Marino, L. Di Matteo, G. De Placido, and A. Izzo Pregnancy after cytoplasmic transfer in a couple suffering from idiopathic infertility: Case report Hum. Reprod., July 1, 2001; 16(7): 1469 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wilding, B. Dale, M. Marino, L. di Matteo, C. Alviggi, M. L. Pisaturo, L. Lombardi, and G. De Placido Mitochondrial aggregation patterns and activity in human oocytes and preimplantation embryos Hum. Reprod., May 1, 2001; 16(5): 909 - 917. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takeuchi, J. Gong, L. L. Veeck, Z. Rosenwaks, and G. D. Palermo Preliminary findings in germinal vesicle transplantation of immature human oocytes Hum. Reprod., April 1, 2001; 16(4): 730 - 736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Barritt, C. A. Brenner, H. E. Malter, and J. Cohen Mitochondria in human offspring derived from ooplasmic transplantation: Brief communication Hum. Reprod., March 1, 2001; 16(3): 513 - 516. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Banerjee, S. Lamond, A. McMahon, S. Campbell, and G. Nargund Does blastocyst culture eliminate paternal chromosomal defects and select good embryos?: Inheritance of an abnormal paternal genome following ICSI Hum. Reprod., December 1, 2000; 15(12): 2455 - 2459. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Van Blerkom, P. Davis, and S. Alexander Differential mitochondrial distribution in human pronuclear embryos leads to disproportionate inheritance between blastomeres: relationship to microtubular organization, ATP content and competence Hum. Reprod., December 1, 2000; 15(12): 2621 - 2633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bahce, T. Escudero, M. Sandalinas, L. Morrison, M. Legator, and S. Munne Improvements of preimplantation diagnosis of aneuploidy by using microwave hybridization, cell recycling and monocolour labelling of probes Mol. Hum. Reprod., September 1, 2000; 6(9): 849 - 854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Tsai, T. Takeuchi, J.M. Bedford, M. M. Reis, Z. Rosenwaks, and G. D. Palermo Alternative sources of gametes: reality or science fiction? Hum. Reprod., May 1, 2000; 15(5): 988 - 998. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tesarik, Z. P. Nagy, C. Mendoza, and E. Greco Chemically and mechanically induced membrane fusion: non-activating methods for nuclear transfer in mature human oocytes Hum. Reprod., May 1, 2000; 15(5): 1149 - 1154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.D. Schramm and B.D. Bavister A macaque model for studying mechanisms controlling oocyte development and maturation in human and non-human primates Hum. Reprod., October 1, 1999; 14(10): 2544 - 2555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Brenner, Y. M. Wolny, R. R. Adler, and J. Cohen Alternative splicing of the telomerase catalytic subunit in human oocytes and embryos Mol. Hum. Reprod., September 1, 1999; 5(9): 845 - 850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Takeuchi, B. Ergun, T. H. Huang, Z. Rosenwaks, and G. D. Palermo A reliable technique of nuclear transplantation for immature mammalian oocytes Hum. Reprod., May 1, 1999; 14(5): 1312 - 1317. [Abstract] [Full Text] [PDF]
|
|