Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (12)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ghetler, Y.
Right arrow Articles by Shalgi, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ghetler, Y.
Right arrow Articles by Shalgi, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction, Vol 4, 289-294, Copyright © 1998 by Oxford University Press

JOURNAL ARTICLE

Cortical granules reaction after intracytoplasmic sperm injection

Y Ghetler, T Raz, I Ben Nun and R Shalgi
IVF Unit, Sapir Medical Center, Kfar Saba, Israel.

The cortical reaction (CR) in mammalian oocytes is induced following sperm-egg membrane fusion. During intracytoplasmic sperm injection (ICSI) the physiological cascade of gamete interaction events is bypassed. The aim of this study was to explore CR occurrence after the ICSI procedure and its correlation with the meiotic status of the oocytes. Unfertilized and abnormally fertilized (one- or three- pronucleate) human oocytes were investigated. The chromosomal status was analysed by Hoechst staining. The CR occurrence and its fluorescent pattern were assessed by confocal scanning laser microscope using the lectin lens culinaris and Texas Red staining. Our results reveal a positive correlation between the activation of oocytes and their CR. No CR was demonstrated in unfertilized-unactivated oocytes with or without sperm in their cytoplasm. In partially activated oocytes showing resumption of meiosis but no formation of pronuclei, a moderate CR was observed. In fully activated oocytes displaying one or three pronuclei, a strong CR was monitored. It was concluded that in ICSI, as in physiological fertilization, oocyte activation is a prerequisite for CR.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
Y. Ghetler, S. Yavin, R. Shalgi, and A. Arav
The effect of chilling on membrane lipid phase transition in human oocytes and zygotes
Hum. Reprod., December 1, 2005; 20(12): 3385 - 3389.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Jimenez-Movilla, M. Aviles, M. J. Gomez-Torres, P. J. Fernandez-Colom, M. T. Castells, J. de Juan, A. Romeu, and J. Ballesta
Carbohydrate analysis of the zona pellucida and cortical granules of human oocytes by means of ultrastructural cytochemistry
Hum. Reprod., August 1, 2004; 19(8): 1842 - 1855.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
F. Miyara, F.-X. Aubriot, A. Glissant, C. Nathan, S. Douard, A. Stanovici, F. Herve, M. Dumont-Hassan, A. L. Meur, P. Cohen-Bacrie, et al.
Multiparameter analysis of human oocytes at metaphase II stage after IVF failure in non-male infertility
Hum. Reprod., July 1, 2003; 18(7): 1494 - 1503.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
P.T. Goud, A.P. Goud, L. Leybaert, P.V. Oostveldt, K. Mikoshiba, M.P. Diamond, and M. Dhont
Inositol 1,4,5-trisphosphate receptor function in human oocytes: calcium responses and oocyte activation-related phenomena induced by photolytic release of InsP3 are blocked by a specific antibody to the type I receptor
Mol. Hum. Reprod., October 1, 2002; 8(10): 912 - 918.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.