Molecular Human Reproduction, Vol 4, 295-300, Copyright © 1998 by Oxford University Press
XQ Wang, ZM Zhu, BA Fenderson, GQ Zeng, YJ Cao and GT Jiang
The role of carbohydrates in embryo implantation in the mouse was
investigated using an embryo transfer model and a blastocyst-uterine
epithelial cell co-culture system. The monoclonal antibody (mAb) AH6
directed to LeY oligosaccharide (Fuc alpha1-2 Gal beta 1-4 [Fuc alpha1- 3]
GlcNAc) and other three mAbs directed to carbohydrates whose structures are
closely related to LeY were used to show the effect of carbohydrate
specificity on implantation. In the embryo transfer model, donor
blastocysts (4 days post-coitus) were pretreated with mAb AH6
(experimental) or other mAbs (control) and transferred into one uterine
horn of a recipient. The implantation rate was checked after 5 days.
Implantation was significantly inhibited by mAb AH6 pretreatment, and
inhibition was not observed in control groups. In the co-culture system,
the attachment and outgrowth rate of blastocysts on the surface of uterine
epithelial cells was significantly inhibited when monolayer epithelial
cells or blastocysts were pretreated with mAb AH6. The most obvious effect
of mAb AH6 was obtained during 2-4 h co-incubation. No inhibition was
observed in the control groups. It was, therefore, concluded that
oligosaccharide LeY recognized by mAb AH6 plays an essential role at the
initial stage of implantation. It may act as a mediator molecule for
adhesion between the surface of blastocyst and epithelial cell, and its
function is carbohydrate-specific.
JOURNAL ARTICLE
Effects of monoclonal antibody directed to LeY on implantation in the mouse
State Key Laboratory of Reproductive Biology, Institute of Zoology, Academia Sinica, Beijing, PRC.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Shi, S. A. Williams, A. Seppo, H. Kurniawan, W. Chen, Z. Ye, J. D. Marth, and P. Stanley Inactivation of the Mgat1 Gene in Oocytes Impairs Oogenesis, but Embryos Lacking Complex and Hybrid N-Glycans Develop and Implant Mol. Cell. Biol., November 15, 2004; 24(22): 9920 - 9929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. E. Domino, L. Zhang, P. J. Gillespie, T. L. Saunders, and J. B. Lowe Deficiency of Reproductive Tract alpha (1,2)Fucosylated Glycans and Normal Fertility in Mice with Targeted Deletions of the FUT1 or FUT2 alpha (1,2)Fucosyltransferase Locus Mol. Cell. Biol., December 15, 2001; 21(24): 8336 - 8345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dettke, G. Pálfi, and H. Loibner Activation-dependent expression of the blood group-related Lewis Y antigen on peripheral blood granulocytes J. Leukoc. Biol., October 1, 2000; 68(4): 511 - 514. [Abstract] [Full Text]
|
|