Molecular Human Reproduction, Vol 4, 301-308, Copyright © 1998 by Oxford University Press
KC Reinhart, RK Dubey, CL Mummery, M van Rooijen, PJ Keller and R Marinella
Leukaemia inhibitory factor (LIF) is an essential factor for embryo
implantation. Factors generated by the oviduct cells (epithelial cells and
fibroblasts) create the microenvironment for fertilization and first embryo
stage development. Hence, it is feasible that the oviduct cells also
synthesize LIF to promote and condition the embryo for implantation in the
uterus. In the present study, we investigated whether cultured bovine
oviduct epithelial cells and fibroblasts synthesize LIF. LIF production was
measured in the conditioned medium of oviduct epithelial cells and
fibroblasts, using LIF enzyme-linked immunosorbent assay. Moreover,
expression of LIF mRNA was confirmed by LIF reverse
transcriptase-polymerase chain reaction in extracts of RNA from oviduct
epithelial/fibroblast cells. Quantitatively similar amounts of LIF were
detected in the culture medium of epithelial cells and fibroblasts. In
cells cultured for 1-7 days, the levels of LIF in the medium increased in a
time-dependent manner. As compared to untreated cells, treatment of cells
with 17beta-oestradiol (1-100 ng/ ml), but not progesterone (1-100 ng/ml)
and insulin (20 ng/ml), increased the levels of LIF in a
concentration-dependent manner (P < 0.05). Similarly, tumour necrosis
factor-alpha (100 ng/ml) significantly induced the levels of LIF. The
effects of 17beta- oestradiol (50 ng/ml) on LIF synthesis were enhanced and
not blocked in the presence of tamoxifen (1 microg/ml), an oestrogen
receptor antagonist, suggesting that the stimulatory effects of 17beta-
oestradiol on LIF synthesis are not receptor-mediated. In conclusion
17beta-oestradiol, but not progesterone, induces LIF synthesis by bovine
oviduct epithelial cells and fibroblasts and this may play an important
role in the biology of early embryo development. However, the exact
pathophysiological role of LIF within the oviduct needs to be further
investigated.
JOURNAL ARTICLE
Synthesis and regulation of leukaemia inhibitory factor in cultured bovine oviduct cells by hormones
Department of Obstetrics and Gynecology, Clinic for Endocrinology, University Hospital, Zurich, Switzerland.
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