Molecular Human Reproduction, Vol 4, 413-421, Copyright © 1998 by Oxford University Press
M Ambhaikar and C Puri
This study demonstrates the presence of [3H]-progesterone binding protein
on the cell surface of human spermatozoa. The binding protein is masked and
is exposed after treatment of spermatozoa with surfactants such as
digitonin. Specific binding of [3H]-progesterone is observed after the
washed spermatozoa or the crude cell membrane fractions are treated with
0.1% digitonin at 0-4 degrees C for 30 min R5020,
17alpha-hydroxyprogesterone and testosterone only partially compete with
[3H]-progesterone for binding whereas the synthetic steroids STS557, RU486,
ZK98.299 and ZK98.734 do not competitively inhibit the binding of
[3H]-progesterone to spermatozoa. Progesterone binding sites are located in
the acrosomal region of spermatozoa, as indicated by the use of fluorescein
isothiocyanate-labelled progesterone-bovine serum albumin. The binding
protein cross-reacts with the monoclonal antibodies to uterine progesterone
receptor and has a molecular weight of approximately 85 kDa. This is the
first study which demonstrates the presence of masked progesterone binding
sites on the cell surface of human spermatozoa that are exposed after
treatment with surfactants. These binding sites seem to have structural
homology with the intracellular progesterone receptor but differ in terms
of ligand specificity and location. The study further demonstrates that
follicular fluid facilitates progesterone binding to spermatozoa.
JOURNAL ARTICLE
Cell surface binding sites for progesterone on human spermatozoa
Institute for Research in Reproduction (ICMR), Parel, Bombay, India.
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