Molecular Human Reproduction, Vol 4, 631-639, Copyright © 1998 by Oxford University Press
CJ Anobile, JA Talbot, SJ McCann, V Padmanabhan and WR Robertson
The heterogeneity of follicle stimulating hormone (FSH) and luteinizing
hormone (LH) was investigated in five women aged 29.4 +/- 3.2 years (mean
+/- SD) throughout their menstrual cycles and in five post- menopausal
women aged 53.8 +/- 5.6 years. Chromatofocusing (pH range 7- 4) revealed
menstrual cycle stage- and postmenopausal-related differences in the serum
gonadotrophin charge. There were differences in the proportion of FSH with
an isoelectric point (pl) > 4.3 across phases of the menstrual cycle (P
= 0.019): midcycle (MC) 50%; early to mid-follicular (EMF) 36%; late
follicular (LF) 37%, luteal (L) 29% and following the menopause (PM) 17%.
There was no significant difference in the proportion of LH with pl >
6.55 between midcycle (53%) and EMF, LF or L phases (36, 43 and 32%
respectively); although all were greater than that found in the menopause
(13%). Concanavalin A chromatography revealed less (P < 0.005) complex
FSH and LH glycoforms at midcycle (63 and 13%) than in the EMF, LF and L
phases (90 and 18; 90 and 20 and 93 and 24% respectively). Menopausal
gonadotrophins were least complex (FSH 34%, LH 4%). There was a direct
relationship between serum FSH and FSH pl/complexity, and less acidic FSH
was associated with reduced FSH complexity. Increased oestradiol was
associated with basic FSH isoforms during the menstrual cycle and reduced
follicular phase FSH complexity. We conclude that changes in gonadotrophin
glycoforms occur through the menstrual cycle which are related to changes
in the prevailing steroid environment. Following the menopause oestrogenic
loss resulted in acidic, relatively simple glycoforms.
JOURNAL ARTICLE
Glycoform composition of serum gonadotrophins through the normal menstrual cycle and in the post-menopausal state
University of Manchester, Department of Medicine, Hope Hospital, Salford, UK.
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