Molecular Human Reproduction, Vol 4, 641-647, Copyright © 1998 by Oxford University Press
JM Rey, P Pujol, H Dechaud, E Edouard, B Hedon and T Maudelonde
Endometrium is one of the main target tissues of oestrogens. Although
homozygous inactivation of oestrogen receptor-alpha leads to infertility in
transgenic mice, oestrogen receptor-alpha is detected in endometrium of
patients with unexplained infertility. Oestrogen receptor-alpha splicing
variants and oestrogen receptor-beta have been studied in oestrogen target
tissues, but their expression in endometrium throughout the menstrual cycle
and in unexplained infertility is unknown. Using reverse
transcription-polymerase chain reaction (RT-PCR), we studied the expression
of oestrogen receptor- alpha splicing variants and oestrogen receptor-beta
in uterine biopsies from 12 patients with endometriosis and 15 patients
with unexplained infertility. A control group included 19 women who had had
a previous pregnancy. Our study gave evidence of exon 2, 3, 4 or 7 deleted
oestrogen receptor-alpha variants co-existing with the wild-type receptor.
We did not find any exon 5 or 6-deleted variants. Exon 4 or 7- deleted
variants were detected in all samples. Exon 2 or 3-deleted variants were
detected at a similar frequency in fertile women (58 and 68% respectively),
endometriotic patients (67 and 83% respectively) and infertile patients (73
and 80% respectively). During the follicular phase, there was a
non-significant trend towards a lower frequency of exon-2 deleted variants
in the fertile group when compared with the hypofertile group. Oestrogen
receptor-beta was detected in all samples. Our preliminary study showed
that altered expression of oestrogen receptor-alpha variants and oestrogen
receptor-beta may not explain the hypofertility state.
JOURNAL ARTICLE
Expression of oestrogen receptor-alpha splicing variants and oestrogen receptor-beta in endometrium of infertile patients
Laboratoire de Biologie Cellulaire et Hormonale, CHRU Arnaud de Villeneuve, Montpellier, France.
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