Molecular Human Reproduction, Vol 4, 667-672, Copyright © 1998 by Oxford University Press
F Saez, C Motta, D Boucher and G Grizard
Prostasomes are human-specific lipid vesicles originating from the prostate
and present in seminal plasma. They are involved in a number of biological
functions such as sperm motility and immunomodulation by seminal plasma.
The aim of our study was to investigate whether prostasomes play a role in
the antioxidant status of seminal plasma. In cell suspensions obtained
after elimination of seminal plasma by centrifugation, reactive oxygen
species (ROS) as measured by luminol- enhanced chemiluminescence were
mainly produced by polymorphonuclear neutrophils (PMN). The addition of
prostasomes to these cell suspensions lowered the overall ROS production in
the basal state and after stimulation with phorbol ester. This action could
not be explained by a ROS-scavenging capacity of the prostasomes, as
demonstrated by their inability to scavenge ROS produced by 2,2'-azobis-
2-amidinopropane dihydrochloride. Using electron spin resonance, we could
assess the influence of prostasomes on the plasma membranes of blood PMN
and show that it was characterized by an increase in the
correlation-relaxation time of the probe 16-doxyl-stearic acid inserted in
the membranes. Thus, prostasomes caused a rigidification of blood PMN
membranes. These results strongly suggest that the effect of prostasomes in
semen could result from their interaction with PMN.
JOURNAL ARTICLE
Antioxidant capacity of prostasomes in human semen
Laboratoire de Biologie de la Reproduction--CECOS, Clermont-Ferrand, France.
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