Molecular Human Reproduction, Vol 4, 709-717, Copyright © 1998 by Oxford University Press
S Yoshioka, H Fujiwara, S Yamada, T Nakayama, T Higuchi, T Inoue, T Mori and M Maeda
We have previously reported that membrane-bound aminopeptidases were
expressed on human follicles and corpora lutea (CL) and we showed that
these aminopeptidases are involved in follicular growth, probably by
regulating extracellular peptide concentrations. In this study, the
expression of membrane-bound carboxypeptidase-M (CP-M), which cleaves
carboxyl-terminal amino acids from peptides extracellularly, on human
follicles and CL was examined. In growing and pre-ovulatory follicles, CP-M
was immunohistochemically detected with weak or moderate intensity on theca
interna cells. Although CP-M was not detected on granulosa cells in growing
and pre-ovulatory follicles, it was strongly detected on the cell surface
of luteinizing granulosa cells isolated from patients undergoing in-vitro
fertilization treatment, indicating that CP-M was rapidly expressed on
granulosa cells during ovulation. In menstrual and pregnant CL, CP-M was
clearly detected on luteal cells. In menstrual CL, the expression of CP-M
mRNA was observed by reverse transcription-polymerase chain reaction
(RT-PCR). Western blotting analysis revealed that the molecular mass of the
CP-M extracted from mid-luteal CL was 62 kDa. These results indicate that
CP-M is a cell surface differentiation-related molecule of human granulosa,
theca, and luteal cells. The rapid expression on granulosa cells during
ovulation strongly suggests the involvement of CP-M in the ovulation and CL
formation processes.
JOURNAL ARTICLE
Membrane-bound carboxypeptidase-M is expressed on human ovarian follicles and corpora lutea of menstrual cycle and early pregnancy
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
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