Molecular Human Reproduction, Vol 4, 803-810, Copyright © 1998 by Oxford University Press
N Hattori, H Fujiwara, M Maeda, S Yoshioka, T Higuchi, T Mori, N Ohishi, M Minami, S Fujii and M Ueda
Leukotriene (LT) A4 hydrolase (EC3.3.2.6) converts LTA4 to LTB4 which shows
a chemotactic activity to leukocytes. To investigate the involvement of
LTB4 in human corpus luteum (CL) function, the localization of LTA4
hydrolase in human ovarian tissues was examined by immunohistochemistry
with a rabbit polyclonal antibody against LTA4 hydrolase. The enzyme was
weakly expressed on granulosa cells of the follicles. After ovulation, the
intensity of LTA4 hydrolase on large luteal cells increased and was highest
in the midluteal phase. High expression was also observed in the CL of
early pregnancy. In theca interna and small luteal cells, LTA4 hydrolase
was weakly detected in all developmental stages. Immunoblot analysis
demonstrated that the molecular mass of LTA4 hydrolase expressed in CL was
62 kDa, and confirmed that LTA4 hydrolase expression increased during CL
formation and remained high in early pregnancy. The sequence encoding mRNA
of LTA4 hydrolase, which was isolated from CL and amplified by polymerase
chain reaction, was shown to be identical to the previously reported one.
Immunocytochemistry showed that LTA4 hydrolase expression in cultured
granulosa cells increased over 4 days in vitro and was enhanced by human
chorionic gonadotrophin treatment. These expression profiles of LTA4
hydrolase suggest the involvement of LTB4 in luteal cell function during CL
formation and early pregnancy.
JOURNAL ARTICLE
Human large luteal cells in the menstrual cycle and early pregnancy express leukotriene A4 hydrolase
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Assou, T. Anahory, V. Pantesco, T. Le Carrour, F. Pellestor, B. Klein, L. Reyftmann, H. Dechaud, J. De Vos, and S. Hamamah The human cumulus-oocyte complex gene-expression profile Hum. Reprod., July 1, 2006; 21(7): 1705 - 1719. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Chen, N. Li, S. Wang, N. Wu, J. Hong, X. Jiao, M. J. Krasna, D. G. Beer, and C. S. Yang Leukotriene A4 Hydrolase in Rat and Human Esophageal Adenocarcinomas and Inhibitory Effects of Bestatin J Natl Cancer Inst, July 16, 2003; 95(14): 1053 - 1061. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Matousek, K. Mitsube, M. Mikuni, and M. Brannstrom Inhibition of ovulation in the rat by a leukotriene B4 receptor antagonist Mol. Hum. Reprod., January 1, 2001; 7(1): 35 - 42. [Abstract] [Full Text] [PDF]
|
|