Molecular Human Reproduction, Vol 4, 811-815, Copyright © 1998 by Oxford University Press
SG Hillier, RA Anderson, AR Williams and M Tetsuka
Ovarian surface epithelial (OSE) cells participate in the formation of the
ovarian cortex and are potential targets of oestrogen action. Oestrogens
typically act through nuclear oestrogen receptors (ER) of which there are
two known subtypes: ERalpha and ERbeta. In view of the potential importance
of oestrogen as a local regulator of OSE cell function, we screened for
ERalpha and ERbeta mRNA in primary OSE cell cultures by reverse
transcriptase-polymerase chain reaction (RT-PCR) analysis, and used freshly
isolated granulosa cells (GC) and granulosa- lutein cells (GLC) as positive
controls. OSE cells, scraped from the ovarian surface of women undergoing
laparotomy for benign gynaecological conditions, were cultured for up to 21
days to obtain enough cells for mRNA extraction. GC were obtained from
spontaneously cyclic women undergoing total hysterectomy; while GLC were
obtained from follicular aspirates of gonadotrophin-stimulated in-vitro
fertilization patients. Total RNA (1 microg) was reverse transcribed into
single-stranded cDNA for PCR (30 cycles) using primers selected to give
specific ERalpha and ERbeta products. The ERalpha and ERbeta PCR products,
authenticated by cloning and sequencing, were both weakly detectable by
Southern analysis in cultured OSE cells and readily detectable in GC and
GLC. These results show that cultured human OSE express both ERalpha and
ERbeta mRNA, consistent with a role for oestrogen in the regulation of OSE
cell function in vivo.
JOURNAL ARTICLE
Expression of oestrogen receptor alpha and beta in cultured human ovarian surface epithelial cells
Department of Obstetrics and Gynaecology, University of Edinburgh Medical School, Centre for Reproductive Biology, UK.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  G. Papacleovoulou, K. Hogg, K. S. Fegan, H. O.D. Critchley, S. G. Hillier, and J. I. Mason Regulation of 3{beta}-hydroxysteroid dehydrogenase type 1 and type 2 gene expression and function in the human ovarian surface epithelium by cytokines Mol. Hum. Reprod., June 1, 2009; 15(6): 379 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Morandi Alessio, L. H. Siqueira, E. C. Couto de Carvalho, R. Barini, A. de Padua Mansur, N. Fenalti Hoehr, and J. M. Annichino-Bizzacchi Estrogen Receptor Alpha and Beta Gene Polymorphisms Are Not Risk Factors for Recurrent Miscarriage in a Brazilian Population Clinical and Applied Thrombosis/Hemostasis, April 1, 2008; 14(2): 180 - 185. [Abstract] [PDF]
|
|
|
|
 |
|
 K S Fegan, M T Rae, H O D Critchley, and S G Hillier Anti-inflammatory steroid signalling in the human peritoneum J. Endocrinol., February 1, 2008; 196(2): 369 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-H. Choi, A. S. T. Wong, H.-F. Huang, and P. C. K. Leung Gonadotropins and Ovarian Cancer Endocr. Rev., June 1, 2007; 28(4): 440 - 461. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. C.K. Leung and J.-H. Choi Endocrine signaling in ovarian surface epithelium and cancer Hum. Reprod. Update, March 1, 2007; 13(2): 143 - 162. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Rae, O. Gubbay, A. Kostogiannou, D. Price, H. O. D. Critchley, and S. G. Hillier Thyroid Hormone Signaling in Human Ovarian Surface Epithelial Cells J. Clin. Endocrinol. Metab., January 1, 2007; 92(1): 322 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O Gubbay, M T Rae, A S McNeilly, F X Donadeu, A J Zeleznik, and S G Hillier cAMP response element-binding (CREB) signalling and ovarian surface epithelial cell survival. J. Endocrinol., October 1, 2006; 191(1): 275 - 285. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Sellers, J. M. Schildkraut, V. S. Pankratz, R. A. Vierkant, Z. S. Fredericksen, J. E. Olson, J. Cunningham, W. Taylor, M. Liebow, C. McPherson, et al. Estrogen Bioactivation, Genetic Polymorphisms, and Ovarian Cancer Cancer Epidemiol. Biomarkers Prev., November 1, 2005; 14(11): 2536 - 2543. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M T Rae, D Niven, A Ross, T Forster, R Lathe, H O D Critchley, P Ghazal, and S G Hillier Steroid signalling in human ovarian surface epithelial cells: the response to interleukin-1{alpha} determined by microarray analysis J. Endocrinol., October 1, 2004; 183(1): 19 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. T. Rae, D. Niven, H. O. D. Critchley, C. R. Harlow, and S. G. Hillier Antiinflammatory Steroid Action in Human Ovarian Surface Epithelial Cells J. Clin. Endocrinol. Metab., September 1, 2004; 89(9): 4538 - 4544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Y.K. Yong, C. Harlow, K.J. Thong, and S. G. Hillier Regulation of 11{beta}-hydroxysteroid dehydrogenase type 1 gene expression in human ovarian surface epithelial cells by interleukin-1 Hum. Reprod., September 1, 2002; 17(9): 2300 - 2306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.B. Dale, S.L. Sarich, T.M. Bretz, J.F. Hatton, and R.J. Zachow Hormonal Regulation of Androgen Receptor Messenger Ribonucleic Acid Expression in Human Tooth Pulp Journal of Dental Research, May 1, 2002; 81(5): 360 - 365. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. W. Paige, K. J. Taylor, C. Taylor, S. G. Hillier, S. Farrington, D. Scott, D. J. Porteous, J. F. Smyth, H. Gabra, and J. E. V. Watson WWOX: A candidate tumor suppressor gene involved in multiple tumor types PNAS, September 25, 2001; 98(20): 11417 - 11422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Cardenas, K.A. Burke, R.M. Bigsby, W.F. Pope, and K.P. Nephew Estrogen Receptor {beta} in the Sheep Ovary During the Estrous Cycle and Early Pregnancy Biol Reprod, July 1, 2001; 65(1): 128 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. Palter, A. B. Tavares, A. Hourvitz, J. D. Veldhuis, and E. Y. Adashi Are Estrogens of Import to Primate/Human Ovarian Folliculogenesis? Endocr. Rev., June 1, 2001; 22(3): 389 - 424. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sundarrajan, W. X. Liao, A. C. Roy, and S. C. Ng Association between Estrogen Receptor-{beta} Gene Polymorphisms and Ovulatory Dysfunctions in Patients with Menstrual Disorders J. Clin. Endocrinol. Metab., January 1, 2001; 86(1): 135 - 139. [Abstract] [Full Text]
|
|
|
|
|
|
P. T.K. Saunders, M. R. Millar, K. Williams, S. Macpherson, D. Harkiss, R. A. Anderson, B. Orr, N. P. Groome, G. Scobie, and H. M. Fraser Differential Expression of Estrogen Receptor-{alpha} and -{beta} and Androgen Receptor in the Ovaries of Marmosets and Humans Biol Reprod, October 1, 2000; 63(4): 1098 - 1105. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Misiti, S. Nanni, G. Fontemaggi, Y.-S. Cong, J. Wen, H. W. Hirte, G. Piaggio, A. Sacchi, A. Pontecorvi, S. Bacchetti, et al. Induction of hTERT Expression and Telomerase Activity by Estrogens in Human Ovary Epithelium Cells Mol. Cell. Biol., June 1, 2000; 20(11): 3764 - 3771. [Abstract] [Full Text]
|
|
|
|
|
|
S. Chu, P. Mamers, H. G. Burger, and P. J. Fuller Estrogen Receptor Isoform Gene Expression in Ovarian Stromal and Epithelial Tumors J. Clin. Endocrinol. Metab., March 1, 2000; 85(3): 1200 - 1205. [Abstract] [Full Text]
|
|
|
|
|
|
C. Sundarrajan, W.-X. Liao, A.C. Roy, and S.C. Ng Association of oestrogen receptor gene polymorphisms with outcome of ovarian stimulation in patients undergoing IVF Mol. Hum. Reprod., September 1, 1999; 5(9): 797 - 802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-M. Lau, S. C. Mok, and S.-M. Ho Expression of human estrogen receptor-alpha and -beta , progesterone receptor, and androgen receptor mRNA in normal and malignant ovarian epithelial cells PNAS, May 11, 1999; 96(10): 5722 - 5727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. Risch RESPONSE - Re: Hormonal Etiology of Epithelial Ovarian Cancer, With a Hypothesis Concerning the Role of Androgens and Progesterone J Natl Cancer Inst, April 7, 1999; 91(7): 650a - 651a. [Full Text] [PDF]
|
|