Molecular Human Reproduction, Vol. 5, No. 10, 920-926,
October 1999
© 1999 European Society of Human Reproduction and Embryology
Regulation of ovarian function |
Co-expression of integrin-associated protein (IAP/CD47) and its ligand thrombospondin-1 on human granulosa and large luteal cells
1 Department of Gynecology and Obstetrics and 2 Clinical Molecular Biology, Faculty of Medicine, and 3 Institute for Frontier Medical Science, Kyoto University, Sakyo-ku, Kyoto 606–01, Japan
Abstract
In the present study, we have raised a monoclonal antibody (mAb) designated OG-4 against human granulosa cells (GC). By immunohistochemistry, the expression of OG-4 antigen was observed on human GC and large luteal cells, but not on thecal and small luteal cells. A complementary DNA (cDNA) clone encoding OG-4 antigen was screened and isolated by a panning method using OG-4 mAb from a human corpus luteum (CL) cDNA library that was expressed transiently in COS-7 cells. Nucleotide sequencing revealed that OG-4 antigen was identical to integrin-associated protein (IAP)/CD47 antigen. Subsequent reverse transcription–polymerase chain reaction (RT–PCR) analysis revealed that the isoform 2 mRNA of IAP is predominantly expressed in human GC and CL. The expression of thrombospondin-1 (TSP-1), which is a ligand for IAP, was also observed in human GC by immunocytochemistry and RT–PCR. Co-expresson of IAP and TSP-1 on human GC may suggest that TSP-1 has a physiological role in GC function possibly via IAP in an autocrine fashion.
granulosa cells/integrin-associated protein/monoclonal antibody/panning/thrombospondin-1
Notes
4 To whom correspondence should be addressed
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