Molecular Human Reproduction, Vol. 5, No. 10, 927-933,
October 1999
© 1999 European Society of Human Reproduction and Embryology
Molecular aspects of oogenesis |
Mitochondrial DNA rearrangements in human oocytes and embryos
1 The Institute for Reproductive Medicine and Science of Saint Barnabas Medical Center, Gamete and Embryo Research Laboratory, Livingston, New Jersey and 2 Medical College of Virginia, Virginia Commonwealth University, Department of Obstetrics and Gynecology, Richmond, VA, USA
Abstract
Human mitochondrial DNA (mtDNA) rearrangements, including more than 150 deletions and insertions, accumulate with age and are responsible for certain neuromuscular diseases. Human oocytes, arrested for up to 50 years, may express certain mtDNA rearrangements possibly affecting function. Investigations have previously shown a single mtDNA rearrangement (dmtDNA4977) in human oocytes. Sequencing of other rearrangements and their correlation with maternal age have not been performed in human oocytes or embryos. Here we use a nested PCR strategy of long followed by short polymerase chain reaction (PCR) that amplifies two-thirds of the mitochondrial genome. mtDNA rearrangements were detected in 50.5% of the oocytes (n = 295) and 32.5% of the embryos (n = 197). This represents a significant difference in the percentage of mtDNA rearrangements between oocytes and embryos (P < 0.0001). Twenty-three novel mtDNA rearrangements with deletions, insertions and duplications were found. There was no significant age-related increase in the percentage of human oocytes or embryos that contained mtDNA rearrangements. Significant reductions in the number of oocytes containing mtDNA rearrangements occurred as oocyte development progressed from germinal vesicle to the mature metaphase II oocyte (P < 0.05). These findings are discussed as they relate to mitochondria, mtDNA, and ATP production in human oocytes and embryos.
DNA rearrangements/embryos/mitochondrial deletions/mitochondrial DNA/oocytes
Notes
3 To whom correspondence should be addressed
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