Molecular Human Reproduction, Vol. 5, No. 10, 950-954,
October 1999
© 1999 European Society of Human Reproduction and Embryology
Molecular events in the endometrium |
Regulation of matrix metalloproteinases (MMPs) and their tissue inhibitors in human myometrial smooth muscle cells by TGF-ß1
Department of Obstetrics and Gynecology, University of Florida, Gainesville, FL 32610, USA
Abstract
The objective of the present study was to determine whether transforming growth factor ß (TGF-ß) regulates the expression of matrix metalloproteinases (MMP) and the tissue inhibitor of MMP (TIMP) in myometrial smooth muscle cells. Using primary cultures of human myometrial smooth muscle cells we found that these cells express MMP-1, MMP-3, TIMP-1 and TIMP-2 mRNA and protein, with significantly higher values of TIMP than MMP. We also found that TGF-ß1 (1 ng/ml) increased the expression of TIMP-1 mRNA, while it reduced the expression of MMP-1 and MMP-3 mRNA, compared with untreated controls. In addition, TGF-ß1 slightly increased the production of TIMP-1, but not TIMP-2. Production of MMP-1 and MMP-3 was reduced by treatment with TGF-ß1, compared with the untreated control. A major portion of MMP-1 released into the culture-conditioned media was in complex with TIMP-1, and the levels of this complex were reduced by treatment with TGF-ß1. In conclusion, the data indicate that myometrial smooth muscle cells express MMP and TIMP mRNA and protein, and their expression is differentially regulated by TGF-ß1. Such a differential regulation of MMP and TIMP by TGF-ß may influence the rate of extracellular matrix (ECM) turnover following tissue injury, induced during myomectomy and Caesarean section, or in leiomyomas during growth.
MMP/myometrial smooth muscle cells/TIMP/transforming growth factor ß (TGF-ß)
Notes
1 To whom correspondence should be addressed
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