Molecular Human Reproduction, Vol. 5, No. 11, 1011-1016,
November 1999
© 1999 European Society of Human Reproduction and Embryology
Regulation of ovarian function |
The involvement of nitric oxide in corpus luteum regression in the rat: feedback mechanism between prostaglandin F2
and nitric oxide
Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Serrano 669, (1414) Buenos Aires, Argentina
Abstract
In the corpus luteum (CL), prostaglandin F2
(PGF2) is a physiological agent with luteolytic actions. Nitric oxide (NO) is a messenger molecule capable of modulating diverse pathophysiological processes. The aim of the present study was to investigate the role of ovarian NO in PGE (a luteotrophic prostanoid) and PGF2 (a luteolytic prostanoid) production and in progesterone synthesis during CL regression in the rat. To obtain a longer functional CL, we used a pseudopregnant (PSP) rat model. By means of intrabursa ovarian sac treatment of two competitive nitric oxide synthase (NOS) inhibitors, NG-monomethyl-l-arginine (l-NMMA, 1 mg/kg) and NW-nitro-l-arginine methyl ester (l-NAME; 3 mg/kg), and sodium nitroprusside (SNP, 0.05 mg/kg) as a NO generator, we found that NO, produced by the ovarian tissue during the last 2 days of CL development (days 8 and 9), increased PGF2 production in the ovary and diminished serum progesterone concentrations leading to CL involution. We also proposed a positive feedback mechanism between PGF2 and NO, to ensure luteal regression. Thus, we injected intraperitoneally a luteolytic dose (3 µg/kg) of a synthetic PGF2 during the mid and late phase of CL development. Ovarian NOS activity was evaluated. The results confirmed our hypothesis; we did not see any effect in the mid-stage of CL development, but increased ovarian NOS activity was found in PGF2-injected late pseudopregnant rats.
corpus luteum/luteolysis/nitric oxide/prostaglandins/rat
Notes
1 To whom correspondence should be addressed
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