Molecular Human Reproduction, Vol. 5, No. 11, 1040-1047,
November 1999
© 1999 European Society of Human Reproduction and Embryology
Molecular events in the uterus |
Localization of nitric oxide synthase and effects of nitric oxide donors on the human Fallopian tube
Department of Obstetrics and Gynaecology, Göteborg University, Sahlgrenska University Hospital, 413 45 Göteborg, Sweden
Abstract
The objective of the study was to assess the plausible existence of a nitric oxide (NO) system within the human Fallopian tube and to examine the effects of NO on tubal contractility. Tissue was obtained from fertile women at operations due to non-tubal diseases. Production of NO and sites of nitric oxide synthase (NOS) activity were assessed by the use of NADPH diaphorase staining and by immunoblots as well as immunohistochemistry for the isoforms of NOS. Effects of NO on tubal contractility in vitro were examined by adding either of two NO donors (nitroglycerin, spermine NONOate) or an analogue of its second messenger (8-bromo cyclic GMP). The production of NO was indicated by positive NADPH diaphorase staining. In immunoblots, endothelial and inducible NOS were demonstrated in all samples analysed. By immunohistochemistry, moderate staining for endothelial NOS was demonstrated in the luminal epithelial cells and in the endothelial cells of blood vessels. Moderate staining for inducible NO synthase was seen in smooth muscle cells and weak staining in epithelial cells. Nitroglycerin, spermine NONOate and 8-bromo cGMP all resulted in a concentration-dependent inhibition of contractility with significant contractility inhibition at 10–7 mol/l, 10–6 mol/l and 10–5 mol/l respectively. The study demonstrates the existence of an endogenous NO system, which may be of physiological importance in Fallopian tube function.
contractile activity/Fallopian tube/human/nitric oxide/nitric oxide synthase
Notes
1 To whom correspondence should be addressed
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