Molecular Human Reproduction, Vol. 5, No. 11, 1055-1058,
November 1999
© 1999 European Society of Human Reproduction and Embryology
Regulation of implantation |
Effects of interleukin-6 (IL-6) on cytotrophoblastic cells
Department of Obstetrics and Gynaecology, Geneva University Hospital and WHO Collaborating Centre in Human Reproduction, University of Geneva, Switzerland
Abstract
Tumour invasion and trophoblastic invasion share the same biochemical mediators: the matrix metalloproteinases (MMP) and their inhibitors. In contrast to tumour invasion of a host tissue, trophoblastic invasion during implantation and placentation is stringently controlled both in tissue localization and developmental stage. The factors responsible for these important regulatory processes are unknown, but in-vitro studies point to endometrial cytokines and growth factors as possible candidates. Here we examined the possibility that interleukin-6 (IL-6), a trophoblastic and endometrial cytokine, represents such a regulatory factor. Purified first trimester cytotrophoblastic cells (CTB) were cultured for 4 days in presence or absence of increasing concentrations of IL-6. MMP-2 and MMP-9 bioactivity (zymography) and immunoactivity were measured in the culture supernatants together with total human chorionic gonadotrophin (HCG), fetal fibronectin (FFN) and leptin. IL-6 did not change the cytotrophoblastic secretion of FFN or total HCG. In contrast, this cytokine induced a dose-dependent stimulation of the leptin secretion and increased the activity, but not the immunoreactivity, of MMP-9 and MMP-2. These results indicate that IL-6 could be considered as an endometrio–trophoblastic regulator of cytotrophoblastic gelatinases.
interleukin-6/leptin/MMP/trophoblastic invasion
Notes
1 To whom correspondence should be addressed at: Laboratoire d'Hormonologie Maternité, 1211 Geneva 14, Switzerland
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Tapia, L. A. Salamonsen, U. Manuelpillai, and E. Dimitriadis Leukemia inhibitory factor promotes human first trimester extravillous trophoblast adhesion to extracellular matrix and secretion of tissue inhibitor of metalloproteinases-1 and -2 Hum. Reprod., August 1, 2008; 23(8): 1724 - 1732. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Dominguez, S. Martinez, A. Quinonero, F. Loro, J.A. Horcajadas, A. Pellicer, and C. Simon CXCL10 and IL-6 induce chemotaxis in human trophoblast cell lines Mol. Hum. Reprod., July 1, 2008; 14(7): 423 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Fitzgerald, T. G. Poehlmann, E. Schleussner, and U. R. Markert Trophoblast invasion: the role of intracellular cytokine signalling via signal transducer and activator of transcription 3 (STAT3) Hum. Reprod. Update, April 17, 2008; (2008) dmn010v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. V. Ingman and R. L. Jones Cytokine knockouts in reproduction: the use of gene ablation to dissect roles of cytokines in reproductive biology Hum. Reprod. Update, March 1, 2008; 14(2): 179 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-H. Zhou, M.-R. Du, L. Dong, X.-Y. Zhu, J.-Y. Yang, Y.-Y. He, and D.-J. Li Cyclosporin A increases expression of matrix metalloproteinase 9 and 2 and invasiveness in vitro of the first-trimester human trophoblast cells via the mitogen-activated protein kinase pathway Hum. Reprod., October 1, 2007; 22(10): 2743 - 2750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-R. Du, W.-H. Zhou, F.-T. Yan, X.-Y. Zhu, Y.-Y. He, J.-Y. Yang, and D.-J. Li Cyclosporine A induces titin expression via MAPK/ERK signalling and improves proliferative and invasive potential of human trophoblast cells Hum. Reprod., September 1, 2007; 22(9): 2528 - 2537. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E Street, P. Seghini, S. Fieni, M. A. Ziveri, C. Volta, D. Martorana, I. Viani, D. Gramellini, and S. Bernasconi Changes in interleukin-6 and IGF system and their relationships in placenta and cord blood in newborns with fetal growth restriction compared with controls. Eur. J. Endocrinol., October 1, 2006; 155(4): 567 - 574. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Leisser, L. Saleh, S. Haider, H. Husslein, S. Sonderegger, and M. Knofler Tumour necrosis factor-{alpha} impairs chorionic gonadotrophin {beta}-subunit expression and cell fusion of human villous cytotrophoblast Mol. Hum. Reprod., October 1, 2006; 12(10): 601 - 609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Scaife, J. N. Bulmer, S. C. Robson, B. A. Innes, and R. F. Searle Effector Activity of Decidual CD8+ T Lymphocytes in Early Human Pregnancy Biol Reprod, October 1, 2006; 75(4): 562 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hirata, T. Sato, M. Tsumagari, A. Shimada, H. Nakano, K. Hashizume, and A. Ito Differential Regulation of the Expression of Matrix Metalloproteinases and Tissue Inhibitors of Metalloproteinases by Cytokines and Growth Factors in Bovine Endometrial Stromal Cells and Trophoblast Cell Line BT-1 In Vitro Biol Reprod, April 1, 2003; 68(4): 1276 - 1281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Cameo, P. Bischof, and J. C. Calvo Effect of Leptin on Progesterone, Human Chorionic Gonadotropin, and Interleukin-6 Secretion by Human Term Trophoblast Cells in Culture Biol Reprod, February 1, 2003; 68(2): 472 - 477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B.A. Cork, E.M. Tuckerman, T.C. Li, and S.M. Laird Expression of interleukin (IL)-11 receptor by the human endometrium in vivo and effects of IL-11, IL-6 and LIF on the production of MMP and cytokines by human endometrial cells in vitro Mol. Hum. Reprod., September 1, 2002; 8(9): 841 - 848. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. A. Odegard, L. J. Vatten, S. T. Nilsen, K. A. Salvesen, H. Vefring, and R. Austgulen Umbilical Cord Plasma Interleukin-6 and Fetal Growth Restriction in Preeclampsia: A Prospective Study in Norway Obstet. Gynecol., August 1, 2001; 98(2): 289 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Brown, D. E. Berkowitz, and M. J. Breslow Weight Loss Is Not Associated with Hyperleptinemia in Humans with Pancreatic Cancer J. Clin. Endocrinol. Metab., January 1, 2001; 86(1): 162 - 166. [Abstract] [Full Text]
|
|
|
|
|
|
M. C. Henson and V. D. Castracane Leptin in Pregnancy Biol Reprod, November 1, 2000; 63(5): 1219 - 1228. [Abstract] [Full Text]
|
|
|
|
|
|
R. Daniels, V. Hall, and A.O. Trounson Analysis of Gene Transcription in Bovine Nuclear Transfer Embryos Reconstructed with Granulosa Cell Nuclei Biol Reprod, April 1, 2000; 63(4): 1034 - 1040. [Abstract] [Full Text]
|
|