Molecular Human Reproduction, Vol. 5, No. 11, 1059-1065,
November 1999
© 1999 European Society of Human Reproduction and Embryology
Regulation of implantation |
Interleukin-10 receptors are expressed by basement membrane anchored, 
6 integrin+ cytotrophoblast cells in early human placenta*
1 Department of Obstetrics and Gynaecology, WHO Collaborative Centre for Research in Human Reproduction, Albert Szent-Györgyi Medical University, Szeged, H-6725, and 2 Department of Dermatology, Albert Szent-Györgyi Medical University, Szeged, H-6720, Hungary
Abstract
Cytotrophoblast cells produce interleukin (IL)-10 and express IL-10 receptor mRNA in culture. Furthermore, IL-10 dramatically reduces the synthesis of matrix metalloproteinase (MMP)-9 and the invasivity of cytotrophoblast cells in vitro, suggesting that an autocrine regulatory role in vivo is also possible. To test this hypothesis we investigated the expression of IL-10 receptor protein by first trimester cytotrophoblasts both in vitro and in situ, using flow cytometry and immunohistochemistry. Flow cytometric analyses demonstrated that 75–80% of cytotrophoblasts are able to bind labelled IL-10, suggesting that these cells possess IL-10 receptors in vitro. Serial sections of early human placentae stained for either 
5 and 6 integrin subunits, or for IL-10 receptors respectively, revealed that placental cytotrophoblasts possess cell surface IL-10 receptors not only in vitro, but also in vivo. IL-10 receptors were present mainly on 6 integrin expressing villous cytotrophoblast cells and on 6-positive cells of invasive cell columns located nearest the villous stroma. Differentiated trophoblasts (i.e. 5-positive cells and villous syncytiotrophoblasts) showed no reactivity. This differential expression of IL-10 receptors suggests that IL-10 might suppress the invasivity of undifferentiated cytotrophoblast cells, in vivo, preserving their non-invasive state in an autocrine manner. The possible involvement in cytotrophoblast proliferation and/or differentiation is also discussed.
cytotrophoblast/IL-10 receptor/integrins/placenta
Notes
* Part of the results described here were presented at the XI Congress of Perinatal Medicine, XIX Alpe Adria Meeting held on 3–4 October 1997, Alsópáhok-Hévíz, Hungary
3 To whom correspondence should be addressed at: Department of Obstetrics and Gynaecology, WHO Collaborative Centre for Research in Human Reproduction, Albert Szent-Györgyi Medical University, H-6725 Szeged, Semmelweis u. 1. Hungary
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. A. White, M. Johansson, C. T. Roberts, A. J. Ramsay, and S. A. Robertson Effect of Interleukin-10 Null Mutation on Maternal Immune Response and Reproductive Outcome in Mice Biol Reprod, January 1, 2004; 70(1): 123 - 131. [Abstract] [Full Text] [PDF]
|
|