Molecular Human Reproduction, Vol. 5, No. 11, 1077-1082,
November 1999
© 1999 European Society of Human Reproduction and Embryology
Molecular aspects of pregnancy |
Modulation of human cytotrophoblastic leptin secretion by interleukin-1
and 17ß-oestradiol and its effect on HCG secretion
1 Department of Obstetrics and Gynecology, Clinic of Infertility and Gynecological Endocrinology – WHO Collaborating Center in Human Reproduction, University Hospital of Geneva, 32 Bvd de la Cluse, 1211 Geneva, 2 Department of Pediatrics, Children University Hospital of Geneva, 6 Rue Willy Donzé, 1211 Geneva, and 3 Division of Endocrinology, Diabetology and Metabolism, Department of Medecine, University Hospital, 1011 Lausanne, Switzerland
Abstract
To investigate the role of leptin during pregnancy, we assessed leptin production by pure cultured human cytotrophoblastic cells (CTB), its regulation by cytokines and 17ß-oestradiol and its effects on human chorionic gonadotrophin (HCG) secretion. Purified CTB from first trimester placenta were incubated in duplicates in the presence or absence of cytokines or 17ß-oestradiol. Medium was harvested on day 2 and the culture stopped on day 4. Results were corrected for protein content of each individual well and expressed as percent of controls per day (mean ± SEM). Basal CTB leptin production was 25.2 ± 2.6 (ng/mg prot). In comparison with controls, leptin production was stimulated to 320 ± 16% (P < 0.0001) and 195 ± 3.2% (P < 0.0004) by 3 and 10 ng/ml of interleukin-1
respectively. 17ß-oestradiol 10–6 to 10–9 mol/l increased basal leptin production 5–9-fold, while 10–5 mol/l had no such effect. Basal CTB HCG secretion was 5722 ± 1055 (mIU/mg prot). There was a dose-dependent leptin-induced increase in HCG secretion (P = 0.0039) reaching a 5-fold increase with a leptin concentration of 1 µg/ml (P < 0.006). Gonadotrophin-releasing hormone (GnRH) 8.5x10–8 mol/l significantly increased HCG secretion to 140 ± 21% of controls (P = 0.031). Cetrorelix (0.1 µg/ml) inhibited leptin-induced HCG secretion (P = 0.0028).
cytokine/GnRH/HCG/leptin/placenta
Notes
4 To whom correspondence should be addressed at: Clinique de Stérilité et d'Endocrinologie Gynécologique, Hôpital Cantonal Universitaire de Genève, 32 Bvd de la Cluse, 1211 Genève 14, Switzerland
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