Molecular Human Reproduction, Vol. 5, No. 12, 1122-1126,
December 1999
© 1999 European Society of Human Reproduction and Embryology
Molecular aspects of spermatogenesis |
Specific expression of heat shock protein HspA2 in human male germ cells
1 Center for Reproduction and Genetics, 2 Department of Obstetrics and Gynecology, 3 Department of Urology of Pundang Je-Saeng General Hospital, Kyungki-do, and 4 Department of Life Science, Sogang University, Seoul, Korea
Abstract
In the mouse, the heat shock protein 70-2 (Hsp70-2) has been found to play a critical role in spermatogenesis. The HspA2 gene is the human homologue of the murine Hsp70-2 gene with 91.7% identity in the nucleotide coding sequence. We examined the expression of HspA2 in human tissues. To detect HspA2 expression, antiserum 2A that was raised against mouse Hsp70-2 and that cross-reacted with human HspA2 protein expressed in Escherichia coli was used. The results of Western blotting indicate that significant HspA2 expression occurs in testes with normal spermatogenesis, whereas only a low amount of HspA2 was expressed in testis with Sertoli cell-only syndrome. Only a small amount of HspA2 was detected in breast, stomach, prostate, colon, liver, ovary, and epididymis. Immunoreactivity to HspA2 was present in spermatocytes and spermatids in the testes with normal spermatogenesis, while immunoreactivity to HspA2 in testis with Sertoli cell-only syndrome was remarkably decreased or inconspicuous over the entire cell. These results demonstrate that the HspA2 protein is highly expressed in human male specific germ cells, suggesting that HspA2 protein may play a specific role during meiosis in human testes as found in the murine model.
heat shock proteins/HspA2/male germ cells/spermatogenesis/testis
Notes
5 To whom correspondence should be addressed at: Center for Reproduction and Genetics, Pundang Je-Saeng General Hospital, Kyungki-do 463–050, Korea
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