Molecular Human Reproduction, Vol. 5, No. 2, 153-161,
February 1999
© 1999 European Society of Human Reproduction and Embryology
Expression of calcium binding protein D-9k messenger RNA in the mouse uterine endometrium during implantation
1 Departments of Gynecology and Obstetrics and 2 Clinical Molecular Biology, Faculty of Medicine, Kyoto University, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606–8507, Japan
To investigate the molecular mechanisms of implantation, we constructed a cDNA library of mouse uteri enriched with pregnancy-induced genes by subtractive hybridization and polymerase chain reaction (PCR). One of the isolated clones was the cDNA for the calcium binding protein D-9k (Cabp9k), which is considered to regulate intracytoplasmic concentration and transport of free calcium ions. Northern blot and in-situ hybridization analyses demonstrated that the Cabp9k mRNA was expressed in the endometrial epithelia, both luminal and glandular, in the uterus at the time of implantation. On pregnancy day 5 it was detected in the luminal, but not in the glandular, epithelia. In the oophorectomized adult mice, progesterone enhanced Cabp9k mRNA expression in the uterus, whereas oestrogen did not. Consistent with this, a nucleotide change was identified in the first intron of mouse Cabp9k gene corresponding to the oestrogen responsive element in the rat Cabp9k gene. Transfer of embryos into the uterine cavity of pseudopregnant mice reduced the expression of Cabp9k mRNA in the glandular epithelium, suggesting that Cabp9k mRNA expression is also regulated by embryonal signal(s). These findings demonstrated that Cabp9k mRNA is expressed in the endometrial epithelia during the implantation period under the control of progesterone and the presence of embryo, and suggest that CaBP9k plays a role in implantation by regulating the local calcium concentrations.
calcium binding protein/cDNA subtraction/embryonal signal/endometrium/oestrogen responsive element
3 To whom correspondence should be addressed
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Choi, H. Seo, M. Kim, and H. Ka Dynamic Expression of Calcium-Regulatory Molecules, TRPV6 and S100G, in the Uterine Endometrium During Pregnancy in Pigs Biol Reprod, December 1, 2009; 81(6): 1122 - 1130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Kondoh, T. Okamoto, T. Higuchi, K. Tatsumi, T. Baba, S. K. Murphy, K. Takakura, I. Konishi, and S. Fujii Stress affects uterine receptivity through an ovarian-independent pathway Hum. Reprod., April 1, 2009; 24(4): 945 - 953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-W. Jeong, K. Y. Lee, I. Kwak, L. D. White, S. G. Hilsenbeck, J. P. Lydon, and F. J. DeMayo Identification of Murine Uterine Genes Regulated in a Ligand-Dependent Manner by the Progesterone Receptor Endocrinology, August 1, 2005; 146(8): 3490 - 3505. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-W. Jung, E.-J. Hong, K.-C. Choi, and E.-B. Jeung Novel Progestogenic Activity of Environmental Endocrine Disruptors in the Upregulation of Calbindin-D9k in an Immature Mouse Model Toxicol. Sci., January 1, 2005; 83(1): 78 - 88. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C Luu, G. Y. Nie, A. Hampton, G.-Q. Fu, Y.-X. Liu, and L. A Salamonsen Endometrial expression of calbindin (CaBP)-d28k but not CaBP-d9k in primates implies evolutionary changes and functional redundancy of calbindins at implantation Reproduction, October 1, 2004; 128(4): 433 - 441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. C. Luu, G. Y. Nie, and L. A. Salamonsen Endometrial calbindins are critical for embryo implantation: Evidence from in vivo use of morpholino antisense oligonucleotides PNAS, May 25, 2004; 101(21): 8028 - 8033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Reese, S. K. Das, B. C. Paria, H. Lim, H. Song, H. Matsumoto, K. L. Knudtson, R. N. DuBois, and S. K. Dey Global Gene Expression Analysis to Identify Molecular Markers of Uterine Receptivity and Embryo Implantation J. Biol. Chem., November 16, 2001; 276(47): 44137 - 44145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G.-Y. Nie, Y. L. Jian Wang, H. Minoura, J. K. Findlay, and L. A. Salamonsen Complex Regulation of Calcium-Binding Protein D9k (Calbindin-D9k) in the Mouse Uterus During Early Pregnancy and at the Site of Embryo Implantation Biol Reprod, January 1, 2000; 62(1): 27 - 36. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. J. Armbrecht, M. A. Boltz, and T. L. Hodam Differences in intestinal calcium and phosphate transport between low and high bone density mice Am J Physiol Gastrointest Liver Physiol, January 1, 2002; 282(1): G130 - G136. [Abstract] [Full Text] [PDF]
|
|