Molecular Human Reproduction, Vol. 5, No. 2, 168-174,
February 1999
© 1999 European Society of Human Reproduction and Embryology
CD9 is involved in invasion of human trophoblast-like choriocarcinoma cell line, BeWo cells
1 Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, 2 Department of Gynecology and Obstetrics, Ohtsu Municipal Hospital, Ohtsu, 3 Institute for Virus Research, Kyoto University, and 4 Institute for Frontier Medical Science, Kyoto University, Sakyo-ku, Kyoto 606, Japan
The CD9 molecule is expressed on human extravillous trophoblasts, which invade the endometrium during implantation and placentation. To elucidate the role of CD9 in trophoblastic function, we investigated the expression of CD9 protein and mRNA in BeWo cells, a human trophoblast-like choriocarcinoma cell line, using immunohistochemistry, Western blotting and reverse transcription–polymerase chain reaction (RT–PCR). When BeWo cells were cultured with anti-CD9 monoclonal antibodies (mAb), their invasion through the extracellular matrices was significantly enhanced in a dose-dependent manner. Cell proliferation and human chorionic gonadotrophin production were unaffected. On the other hand, culture in the presence of mAb against integrins 
3, 5 and ß1, which partially block the interaction with the extracellular matrices, inhibited BeWo cell invasion. Anti-CD9 monoclonal antibody had a stimulatory effect on BeWo cell invasion in the presence of anti-integrin 3 antibody. In contrast, it had no effect in the presence of mAb against integrins 5 and ß1, which were also highly expressed on BeWo cells. These findings suggest that CD9 has a function connected with the invasive properties of BeWo cells, which is partially mediated by integrin 5ß1. This may relate to the involvement of CD9 in trophoblastic invasion.
BeWo cell/CD9/invasion/trophoblast
5 To whom correspondence should be addressed at: Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Sakyo-ku, Kyoto 606, Japan
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