Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (26)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Verlinsky, Y.
Right arrow Articles by Evsikov, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Verlinsky, Y.
Right arrow Articles by Evsikov, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction, Vol. 5, No. 2, 89-95, February 1999
© 1999 European Society of Human Reproduction and Embryology

Karyotyping of human oocytes by chromosomal analysis of the second polar bodies

Yury Verlinsky1 and Sergei Evsikov

Reproductive Genetics Institute, 836 W. Wellington Avenue, Chicago, IL 60657, USA

This paper describes a method for obtaining metaphase chromosomes from human second polar bodies. The second polar body nucleus was injected into the cytoplasm of an enucleated oocyte, which is activated shortly after injection. When the polar body nucleus is transformed into a haploid pronucleus, treatment with okadaic acid was used to induce premature chromosome condensation. A total of 25 analysable chromosome plates were obtained from 38 polar bodies karyotyped using this technique. Whole chromosome painting was used to detect second polar bodies (and respectively, oocytes) with unbalanced translocations. In combination with the first polar body analysis, this technique may be useful in preimplantation genetic diagnosis for patients carrying maternal translocations.

metaphase chromosomes/micromanipulations/oocyte activation/polar body/whole chromosome painting

1 To whom correspondence should be addressed


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
L. Gianaroli, M.C. Magli, A.P. Ferraretti, S. Munne, B. Balicchia, T. Escudero, and A. Crippa
Possible interchromosomal effect in embryos generated by gametes from translocation carriers
Hum. Reprod., December 1, 2002; 17(12): 3201 - 3207.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
ESHRE PGD Consortium Steering Committee
ESHRE Preimplantation Genetic Diagnosis Consortium: data collection III (May 2001)
Hum. Reprod., January 1, 2002; 17(1): 233 - 246.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
M. Bahce, T. Escudero, M. Sandalinas, L. Morrison, M. Legator, and S. Munne
Improvements of preimplantation diagnosis of aneuploidy by using microwave hybridization, cell recycling and monocolour labelling of probes
Mol. Hum. Reprod., September 1, 2000; 6(9): 849 - 854.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.