Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Cataldo, L.
Right arrow Articles by Kleene, K. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cataldo, L.
Right arrow Articles by Kleene, K. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction, Vol. 5, No. 3, 206-213, March 1999
© 1999 European Society of Human Reproduction and Embryology

A quantitative sucrose gradient analysis of the translational activity of 18 mRNA species in testes from adult mice

Leah Cataldo, Mary-Ann Mastrangelo and Kenneth C. Kleene1

Department of Biology, University of Massachusetts Boston, 100 Morrissey Blvd, Boston, MA 02125–3393, USA

Sucrose gradients have been widely used to study the translational activity of mRNA species in meiotic and haploid spermatogenic cells in mammals. Unfortunately, the results of these studies have been very inconsistent. The purpose of the present study was to obtain accurate and reproducible measurements of the translational activity of a large number of testicular mRNA in sucrose gradients. Extracts of adult testes and cultured seminiferous tubules were sedimented on sucrose gradients, and the distribution of 18 mRNA species was quantified by phosphoimaging. The proportions of various mRNA species sedimenting with polysomes in meiotic and haploid cells (~6–74%) is less than typical of efficiently translated mRNAs (85–90%), demonstrating that the initiation of translation of virtually all mRNA species is at least partially inhibited and that the extent of inhibition is mRNA-specific. Most mRNA species in meiotic and early haploid spermatogenic cells are translated on polysomes in which the ribosome spacing is somewhat wider than in somatic cells, 100–150 verses 80–100 bases. However, the ribosome spacing on protamine mRNAs is unusually close (40–50 bases), and the spacing on poly(A) binding protein mRNA is unusually wide (212–272 bases), thus suggesting that the rate of translational initiation, termination and/or elongation is regulated on translationally active forms of certain mRNA.

spermatids/spermatocytes/translational regulation

1 To whom correspondence should be addressed


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
M. P. Paronetto, F. Zalfa, F. Botti, R. Geremia, C. Bagni, and C. Sette
The Nuclear RNA-binding Protein Sam68 Translocates to the Cytoplasm and Associates with the Polysomes in Mouse Spermatocytes
Mol. Biol. Cell, January 1, 2006; 17(1): 14 - 24.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Arava, Y. Wang, J. D. Storey, C. L. Liu, P. O. Brown, and D. Herschlag
Genome-wide analysis of mRNA translation profiles in Saccharomycescerevisiae
PNAS, April 1, 2003; 100(7): 3889 - 3894.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Y. Iuchi, T. Kaneko, S. Matsuki, I. Sasagawa, and J. Fujii
Concerted Changes in the YB2/RYB-a Protein and Protamine 2 Messenger RNA in the Mouse Testis under Heat Stress
Biol Reprod, January 1, 2003; 68(1): 129 - 135.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
J. P. Siffroi, A. Pawlak, M. F. Alfonsi, F. Troalen, G. Guellaen, and J. P. Dadoune
Expression of the TAR RNA binding protein in human testis
Mol. Hum. Reprod., March 1, 2001; 7(3): 219 - 225.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
M.-A. Mastrangelo and K. C. Kleene
Developmental expression of Y-box protein 1 mRNA and alternatively spliced Y-box protein 3 mRNAs in spermatogenic cells in mice
Mol. Hum. Reprod., September 1, 2000; 6(9): 779 - 788.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.