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Molecular Human Reproduction, Vol. 5, No. 3, 240-245, March 1999
© 1999 European Society of Human Reproduction and Embryology

Interleukin-1 receptor-ligand interactions modulate interstitial collagenase-1 production by human endometrial fibroblasts

C.F. Singer1,3, E. Marbaix1,2, P. Lemoine1, Y. Eeckhout1 and P.J. Courtoy1,4

1 Cell Biology Unit, Christian de Duve Institute of Cellular Pathology, Avenue Hippocrate 75 and 2 Department of Pathology, Saint-Luc University Clinics, Louvain University Medical School, Avenue Hippocrate 10, B-1200 Brussels, Belgium

The expression of interstitial collagenase-1 in the cycling human endometrium is restricted to the perimenstrual phase and is a key event for matrix degradation that initiates menstruation. In the absence of ovarian steroids, collagenase production by endometrial fibroblasts is induced by epithelial cell-derived interleukin-1{alpha}. Media conditioned by endometrial epithelial cells were found to contain interleukin-1{alpha} but not interleukin-1ß, and their capacity to induce collagenase production by endometrial fibroblasts correlated with interleukin-1{alpha} concentration in a saturable manner. Collagenase induction by recombinant interleukin-1{alpha} was severely inhibited by interleukin-1 receptor antagonist alone and abolished by its combination with soluble interleukin-1 type-II receptor. By contrast, the association of the receptor antagonist with soluble type-I receptor was less effective than each factor alone. Induction of collagenase by epithelial cell-conditioned media was severely inhibited by neutralizing interleukin-1{alpha} antibodies, whereas the combination of receptor antagonist with soluble type-II receptor proved less effective. We conclude that the collagenase response of endometrial fibroblasts to epithelial cell-derived interleukin-1{alpha} is effectively blocked in vitro by soluble members of the interleukin-1 family and can thus be modulated in vivo by these or other local factors.

endometrium/IL-1 receptor antagonist/interleukin-1/interstitial collagenase-1/soluble IL-1 receptor

3 Present address: Division of Special Gynaecology, Department of Obstetrics and Gynaecology, University of Vienna Medical Center, Waehringer Guertel 18–20, A-1090 Vienna, Austria

4 To whom correspondence should be addressed


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