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Molecular Human Reproduction, Vol. 5, No. 3, 261-269, March 1999
© 1999 European Society of Human Reproduction and Embryology

Binding of interleukin-8 to heparan sulphate enhances cervical maturation in rabbits

Hossain Md Belayet1, Naohiro Kanayama, Selina Khatun, Kazuhiro Sumimoto, Takao Kobayashi and Toshihiko Terao

Department of Obstetrics and Gynecology, Hamamatsu University School of Medicine, 3600 Handa Cho, Hamamatsu City 431-3192, Shizuoka Prefecture, Japan

Cervical ripening is a cytokine-trigged process with substantial remodelling of the cervical extracellular matrix. Interleukin-8 (IL-8) is an important cytokine in cervical maturation. Glycosaminoglycans are also included in this process, but their role in not clearly understood. The effects of heparan sulphate (HS), hyaluronic acid (HA), IL-8, HS + IL-8 and HA + IL-8 on biochemical properties of the cervix were examined in non-pregnant rabbits. The changes in vascular pattern with collagen structure of the cervices and immunohistochemical studies, together with the relative collagen concentrations, were determined. A reduction in relative collagen concentration was significant after HS + IL-8 , IL-8 and HA + IL-8 treatment (all P < 0.0001). Gel electrophoresis analysis showed that IL-8 bound preferentially to HS than to HA. Neutrophils were significantly increased in number (P < 0.0001) and located predominantly beneath the glandular epithelium and around the blood vessels after HS + IL-8 treatment. HS + IL-8 treatment caused cervices to increase their water content and become oedematous. The collagen fibres were considerably dissociated, the interfibrillar spaces markedly dilated, and the blood vessels notably increased and dilated. We conclude that binding to HS enhances the activity of IL-8 in inducing cervical maturation.

cervical maturation/heparan sulphate/hyaluronic acid/interleukin-8/neutrophil activation

1 To whom all correspondence should be addressed


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