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Molecular Human Reproduction, Vol. 5, No. 6, 495-497, June 1999
© 1999 European Society of Human Reproduction and Embryology

Existence of human DAZLA protein in the cytoplasm of human oocytes

Shinya Nishi1, Nobuhiko Hoshi1,3, Masanori Kasahara2, Teruo Ishibashi2 and Seiichiro Fujimoto1

1 Department of Obstetrics and Gynecology, Hokkaido University School of Medicine, Sapporo, and 2 Department of Biochemistry, Hokkaido University School of Medicine, Sapporo, Japan

The human deleted in azoospermia-like autosomal (DAZLA) gene is thought to be a candidate gene for azoospermia. cDNA encoding the C-terminal 94 amino acid residues of human DAZLA was used to express a bacterial fusion protein which was then used to raise polyclonal antibodies in rabbits. Immunohistochemical analyses with the human DAZLA antiserum showed that the DAZLA protein is expressed at a cytoplasmic location in female germ cells. Available evidence suggests that the DAZLA gene is a participant in human oogenesis.

DAZLA/immunohistochemistry/oocyte/oogenesis

3 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Hokkaido University School of Medicine, N15 W7, Kita-ku, Sapporo 060–8638, Japan


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