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Molecular Human Reproduction, Vol. 5, No. 6, 527-533, June 1999
© 1999 European Society of Human Reproduction and Embryology

Male accessory sex gland secretions affect oocyte Ca2+ oscillations during in-vitro fertilization in golden hamsters

Y. Ying1, P.H. Chow2, M.P.L. Cheung1 and W.S. O1,3

1 Department of Anatomy, Faculty of Medicine, The University of Hong Kong, 5 Sassoon Road, Hong Kong and 2 Department of Anatomy, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong, China

To evaluate the effect of male accessory sex gland secretions on Ca2+ oscillations of oocytes, epididymal or ejaculated spermatozoa recovered from uteri were used to inseminate oocytes. Ca2+ oscillations were measured by Fura 2 fluorescence imaging (F340/F380). We showed that although Ca2+ oscillations induced by ejaculated spermatozoa had a pattern similar to those induced by epididymal spermatozoa, the amplitude of the first Ca2+ transient in the former group was significantly higher (P < 0.05) and the duration was significantly longer (P < 0.01). Oocytes inseminated with ejaculated spermatozoa recovered from uteri from males had ampullary glands or ventral prostates removed showed significantly lower Ca2+ oscillations compared to the controls (P < 0.05, P < 0.01 respectively). Moreover, the relative area of the first Ca2+ transient in treatment groups was significantly smaller than the control. In addition, a significantly higher percentage of oocytes (52%) inseminated by spermatozoa from males with all accessory sex glands removed showed non-oscillatory Ca2+ transients, compared to the controls (5%, P < 0.05). These results indicate that accessory sex gland secretions can affect Ca2+ oscillations. The differences between Ca2+ oscillations induced by epididymal and uterine spermatozoa from males with all accessory sex glands removed suggest that uterine factors may also influence this process.

Ca2+ oscillations/golden hamster/in-vitro fertilization/male accessory sex glands/oocyte

3 To whom correspondence should be addressed


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