Molecular Human Reproduction, Vol. 5, No. 6, 559-564,
June 1999
© 1999 European Society of Human Reproduction and Embryology
Oestrogen receptor 
and ß mRNA expression in human endometrium throughout the menstrual cycle
1 Department of Obstetrics & Gynecology and 2 Department of Pathology, Tohoku University School of Medicine, 1–1, Seiryo-machi, Aoba-ku, Sendai, 980–8574, Japan
We examined the localization of oestrogen receptor (ER) ß mRNA in the human endometrium throughout the menstrual cycle using non-radioactive in-situ hybridization with Brigati-tailed oligonucleotides. The findings were compared with those of ER
in order to examine the possible biological significance of ERß in the human endometrium. Both ER and ERß mRNA expression were detected in all major human uterine cell types, including glandular epithelial cells, stromal cells and smooth muscle cells of the uterine wall, at every menstrual cycle stage. However, ER mRNA expression was more prominent than that of ERß in all cell types throughout the menstrual cycle. In proliferative phase endometrium, ER mRNA was expressed in both glandular epithelial and stromal cells, while ERß mRNA was expressed predominantly in glandular epithelial cells. Although the same pattern was observed in the secretory phase, both the ER and ERß mRNA expression was relatively weaker. These results suggest that oestrogenic effects occur predominantly through ER, but that ERß may also play a role in the modulation of oestrogenic action, especially on glandular epithelial cells in the human endometrium throughout the menstrual cycle.
endometrium/ER/ERß/in-situ hybridization
3 To whom correspondence should be addressed
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