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Molecular Human Reproduction, Vol. 5, No. 7, 675-681, July 1999
© 1999 European Society of Human Reproduction and Embryology

Production and characterization of monoclonal antibodies against pregnancy-associated plasma protein A

N.A. Bersinger1,4, A. Meisser2, T. Bessou3, P. Seguin3, M.H. Birkhäuser1 and P. Bischof2

1 Department of Obstetrics and Gynaecology, University of Berne, Schanzeneckstrasse 1, Berne CH-3012, 2 Department of Obstetrics and Gynaecology, University of Geneva, Switzerland, 3 CIS-Bio international, In Vitro Technologies Division, Bagnols-sur-Cèze, France

Pregnancy-associated plasma protein A (PAPP-A) was found to be a good first trimester maternal serum marker, together with free ß-human chorionic gonadotrophin (HCG) subunits, for the biochemical screening of fetal trisomy 21 (Down's syndrome). We have raised monoclonal antibodies (mAbs) against PAPP-A purified from human pregnancy serum. The different antibodies were characterized biochemically by Western blot analysis and in terms of specificity (reaction with non-pregnant and male serum). Their performance in Down's syndrome screening was assessed in comparison with an existing enzyme-linked immunosorbent assay method after labelling of the different mAbs with biotin or horseradish peroxidase. A pair of mAbs was eventually chosen for a double-antibody sandwich protocol. The selected combination was found to have a significantly increased specificity (P = 0.0116) over the method using (purified) polyclonal antibodies, together with slightly increased sensitivity. In our limited number of Down's syndrome pregnancy samples (n = 17) and controls (n = 18), the medians as well as the multiples of the median values (for the affected cases) were comparable between the two methods described.

Down's syndrome/monoclonal antibodies/pregnancy-associated plasma protein A (PAPP-A)

4 To whom correspondence should be addressed


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Q.-P. Qin, S. Kokkala, J. Lund, N. Tamm, X. Qin, M. Lepantalo, and K. Pettersson
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