Molecular Human Reproduction, Vol. 5, No. 8, 703-707,
August 1999
© 1999 European Society of Human Reproduction and Embryology
Evidence for the presence of hepatocyte growth factor expression in human ovarian follicles
1 Department of Obstetrics and Gynecology, University of Tokyo, Tokyo 113-8655, and 2 CREST, Japan Science and Technology, Kawaguchi 332-0012, Japan
The presence of hepatocyte growth factor (HGF) in follicular fluid (FF) relative to concentrations of sex steroid hormones and human chorionic gonadotrophin (HCG) was investigated. A total of 69 FF samples were obtained during oocyte retrieval for in-vitro fertilization (IVF) from 11 patients with no apparent endocrine disorders. The concentrations of HGF, oestradiol, progesterone, HCG and testosterone in FF samples were measured by enzyme-linked immunosorbent assay. Transcription of HGF and its receptor, c-met, was detected by reverse transcription–polymerase chain reaction (RT–PCR). Human FF samples contained ~90-fold higher amounts of HGF (24.2 ± 1.2 ng/ml), compared with those of serum (0.28 ± 0.04 ng/ml). Concentrations of HGF in FF were positively correlated with those of progesterone (r = 0.649, P < 0.0001) and HCG (r = 0.264, P = 0.026) concentrations in FF. However, HGF concentrations were not significantly correlated with oestradiol and testosterone. HGF in FF was detected by Western blotting, as a single 90 kDa band, corresponding to a single chain form. Additionally, mRNA for both HGF and its receptor were detected in a crude granulosa cell preparation from the pre-ovulatory follicles. These findings suggest that HGF is produced locally in human ovarian follicles and may have a physiological role as an autocrine/paracrine factor.
follicular fluid/hepatocyte growth factor/IVF embryo transfer/ovary
3 To whom correspondence should be addressed
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Zachow and M. Uzumcu The hepatocyte growth factor system as a regulator of female and male gonadal function J. Endocrinol., December 1, 2007; 195(3): 359 - 371. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Uzumcu, Z. Pan, Y. Chu, P. E Kuhn, and R. Zachow Immunolocalization of the hepatocyte growth factor (HGF) system in the rat ovary and the anti-apoptotic effect of HGF in rat ovarian granulosa cells in vitro. Reproduction, August 1, 2006; 132(2): 291 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Hirota, Y. Osuga, O. Yoshino, K. Koga, T. Yano, T. Hirata, E. Nose, T. Ayabe, A. Namba, O. Tsutsumi, et al. Possible Roles of Thrombin-Induced Activation of Protease-Activated Receptor 1 in Human Luteinized Granulosa Cells J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3952 - 3957. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.B. Dale, S.L. Sarich, T.M. Bretz, J.F. Hatton, and R.J. Zachow Hormonal Regulation of Androgen Receptor Messenger Ribonucleic Acid Expression in Human Tooth Pulp J. Dent. Res., May 1, 2002; 81(5): 360 - 365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Koga, Y. Osuga, O. Tsutsumi, M. Momoeda, A. Suenaga, K. Kugu, T. Fujiwara, Y. Takai, T. Yano, and Y. Taketani Evidence for the Presence of Angiogenin in Human Follicular Fluid and the Up-Regulation of Its Production by Human Chorionic Gonadotropin and Hypoxia J. Clin. Endocrinol. Metab., September 1, 2000; 85(9): 3352 - 3355. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. J. Zachow, B. E. Ramski, and H. Lee Modulation of Estrogen Production and 17{beta}-Hydroxysteroid Dehydrogenase-Type 1, Cytochrome P450 Aromatase, c-Met, and Protein Kinase B{alpha} Messenger Ribonucleic Acid Content in Rat Ovarian Granulosa Cells by Hepatocyte Growth Factor and Follicle-Stimulating Hormone Biol Reprod, June 1, 2000; 62(6): 1851 - 1857. [Abstract] [Full Text]
|
|