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Molecular Human Reproduction, Vol. 5, No. 8, 784-787, August 1999
© 1999 European Society of Human Reproduction and Embryology

Successful metaphase chromosome analysis of human elongated spermatids using mouse oocytes

Y. Araki1,5, S. Ogawa2, M. Ohno1, M. Yoshizawa3, M. Motoyama1, S. Araki2, I. Aslam4 and S. Fishel4

1 The Institute of Advanced Medical Technology Central Clinic, Yakushiji 3154, Minamikawachi, Kawachi, Tochigi 329-0431, 2 Department of Obstetrics and Gynecology, Jichi Medical University, Yakushiji 3153, Minamikawachi, Kawachi, Tochigi 329-0431, 3 Department of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University, Utsunomiya 321-8505, Japan, and 4 CARE (Centres for Assisted Reproduction), The Park Hospital, Sherwood Lodge Drive, Burntstump Country Park, Arnold, Nottingham NG5 8RX, UK

Human elongated spermatids from azoospermic patients were inserted into mouse oocytes by intracytoplasmic sperm injection (ICSI). The injection resulted in survival rates of 46.5% (180 out of 387) and activation rates of 36.1% (65 out of 180). The rate of two pronuclear (2PN) formation was 35.4% (23 out of 65). Only 34.8% (eight out of 23) metaphase chromosome spreads from 2PN zygotes could be analysed; however, all were of normal karyotype. Cytogenetic analysis at the first metaphase revealed that human elongated spermatid chromosomes were able to undergo replication in a heterogeneous environment.

azoospermia/chromosome karyotype/elongated spermatid/G-banding/ICSI

5 To whom correspondence should be addressed


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Y. Araki, M. Yoshizawa, and Y. Araki
A novel method for chromosome analysis of human sperm using enucleated mouse oocytes
Hum. Reprod., May 1, 2005; 20(5): 1244 - 1247.
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