Molecular Human Reproduction, Vol. 5, No. 9, 825-830,
September 1999
© 1999 European Society of Human Reproduction and Embryology
Molecular aspects of spermatogenesis |
Relationship between abnormal sperm chromatin packing and IVF results
1 Department of Molecular Radiobiology and Biophysics, St Petersburg Nuclear Physics Institute Rassian AS, 188350, Gatchina, and 2 D.O.Ott Institute Obstetric and Gynecology Rassian AMS, Mendeleyevskaya Linia 3, 199034, St Petersburg, Russia
Abstract
This study was initiated to determine the relationship between the fertilizing potential of spermatozoa and abnormalities in the compact packing of their chromatin which occurs in the final stage of male germ cell differentiation. Chromatin packing involves disulphide bridge covalent cross-linking. The degree of packing was determined from the accessibility of DNA to a fluorescent dye, ethidium bromide, following detergent treatment of the spermatozoa. The amount of dye bound was determined by flow cytometry in the presence or absence of heparin, a polyanion which removes only non-disulphide bridge-linked proteins. The results of a number of different sperm samples were compared with their results following in-vitro fertilization, and a relationship between disordered sperm chromatin packing and rates of embryo cleavage was observed. This study suggests that abnormal chromatin packing in spermatozoa may contribute to male fertility.
chromatin packing/ethidium bromide/fertility disorder/flow cytometry/human spermatozoa
Notes
3 To whom correspondence should be addressed
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