Molecular Human Reproduction, Vol. 6, No. 1, 68-74,
January 2000
© 2000 European Society of Human Reproduction and Embryology
Uterus and pregnancy |
Identification of carbonic anhydrase XII as the membrane isozyme expressed in the normal human endometrial epithelium
1 Department of Anatomy and Cell Biology, University of Oulu, Box 5000, FIN-90401 Oulu, 2 Department of Clinical Chemistry, University of Oulu, FIN-90401 Oulu, Finland, 3 Department of Internal Medicine, University of Saarland, 66421 Homburg, Germany, 4 Edward A.Doisy Department of Biochemistry and Molecular Biology, St Louis University School of Medicine, St Louis, MO 63104, USA and 5 Department of Obstetrics and Gynecology, University of Oulu, FIN-90401 Oulu, Finland
Abstract
Although previous studies demonstrated carbonic anhydrase (CA) activity in the human endometrium, the CA isozyme(s) responsible for this activity has not been established. In this report, we provide the first evidence that the CA isozyme XII, a recently identified transmembrane isozyme that is expressed in normal kidney and greatly overexpressed in some renal cancers, is present in endometrium. We show by immunohistochemistry that CA XII is expressed in the basolateral plasma membrane of epithelial cells of normal human endometrium. Expression of CA XII in uterus was confirmed by Northern blotting. Detergent-solubilized CA XII was isolated from human endometrium by inhibitor affinity chromatography and characterized by isoelectric focusing and Western blot as a polypeptide with a pI of 6.3. The high expression of CA XII in the endometrial epithelium suggests that it may be functionally linked to the pH-dependent events in spermatozoa that precede fertilization. Its basolateral location and extracellular active site could also allow it to influence the morphological changes in endometrium that occur during the menstrual cycle.
bicarbonate/carbonic anhydrase/immunohistochemistry/pH/uterus
Notes
6 To whom correspondence should be addressed
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