Molecular Human Reproduction, Vol. 6, No. 1, 96-102,
January 2000
© 2000 European Society of Human Reproduction and Embryology
Uterus and pregnancy |
Up-regulation of matrix metalloproteinase-9 in human myometrium during labour: a cytokine-mediated process in uterine smooth muscle cells
Department of Obstetrics and Gynecology, Samsung Medical Center, and Center for Clinical Research, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Seoul, 135–710, Korea
Abstract
The pregnant uterus undergoes dramatic changes of tissue remodelling during the labour and post-partum period. We studied the production of matrix metalloproteinase 9 (MMP-9), as a major contributor of tissue remodelling, in human myometrium at parturition. The regulation of proMMP-9 by interleukin-1ß (IL-1ß) and tumour necrosis factor-
(TNF-) was also investigated in human myometrial smooth muscle cells. MMP-9 was present in myometrial smooth muscle cells, interstitial fibroblasts and inflammatory cells. The gelatinolytic activities of proMMP-9 in myometrium increased dramatically during labour. IL-1ß and TNF- induced proMMP-9 in myometrial smooth muscle cells, but these effects did not seem to be mediated by protein kinase C. On the other hand, neither 17ß-oestradiol nor progesterone itself affected proMMP-9 production in myometrial smooth muscle cells. Moreover, progesterone, which is known as a physiological suppressor of MMP-9 in other species, did not decrease the IL-1ß- and TNF--induced production of proMMP-9. These results suggest that IL-1ß and TNF- are effective up-regulators of proMMP-9 in the tissue remodelling of human myometrium during labour.
interleukin-1ß/matrix metalloproteinase-9/myometrium/smooth muscle cell/tumour necrosis factor-
Notes
1 To whom correspondence should be addressed
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