Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Tesarik, J.
Right arrow Articles by Greco, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Tesarik, J.
Right arrow Articles by Greco, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction, Vol. 6, No. 10, 877-881, October 2000
© 2000 European Society of Human Reproduction and Embryology

Testis and spermatogenesis

The effect of FSH on male germ cell survival and differentiation in vitro is mimicked by pentoxifylline but not insulin

Jan Tesarik1,2,5, Carmen Mendoza2,3 and Ermanno Greco4

1 Laboratoire d'Eylau, 55 rue Saint-Didier, 75116 Paris, France, 2 MAR&Gen, Molecular Assisted Reproduction and Genetics, Gracia 36, 18002 Granada, Spain, 3 University of Granada, Campus Universitario Fuentenueva, 18004 Granada, Spain and 4 European Hospital, Via Portuense 700, Rome, Italy

Abstract

High concentrations of FSH have been shown to boost in-vitro differentiation of germ cells from men with normal spermatogenesis and from some patients with in-vivo maturation arrest. This study shows that the differentiation-promoting effect of FSH is connected to protection against germ cell apoptosis and that both effects can be mimicked by the intracellular cyclic AMP (cAMP)-elevating drug pentoxifylline. On the other hand, a high concentration of insulin, supposed to act at the insulin-like growth factor I receptor, did not exert any effect either on differentiation or apoptosis of germ cells in vitro. These data show that the in-vitro effects of supraphysiological concentrations of FSH on human spermatogenesis are mediated by the classical FSH signal transduction pathway involving cAMP as a second messenger. Pentoxifylline may thus be useful as an alternative means for intracellular cAMP elevation in men with high circulating FSH concentrations leading to desensitization of the FSH receptor.

FSH/in-vitro spermatogenesis/insulin/male germ cell/pentoxifylline

Notes

5 To whom correspondence should be addressed at: Laboratoire d'Eylau, 55 rue Saint-Didier, 75116 Paris.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
A. Feki, C.-E. Jefford, P. Durand, J. Harb, H. Lucas, K.-H. Krause, and I. Irminger-Finger
BARD1 Expression During Spermatogenesis Is Associated with Apoptosis and Hormonally Regulated
Biol Reprod, November 1, 2004; 71(5): 1614 - 1624.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. Tesarik, F. Martinez, L. Rienzi, M. Iacobelli, F. Ubaldi, C. Mendoza, and E. Greco
In-vitro effects of FSH and testosterone withdrawal on caspase activation and DNA fragmentation in different cell types of human seminiferous epithelium
Hum. Reprod., July 1, 2002; 17(7): 1811 - 1819.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
K. Erkkila, H. Aito, K. Aalto, V. Pentikainen, and L. Dunkel
Lactate inhibits germ cell apoptosis in the human testis
Mol. Hum. Reprod., February 1, 2002; 8(2): 109 - 117.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Sousa, N. Cremades, C. Alves, J. Silva, and A. Barros
Developmental potential of human spermatogenic cells co-cultured with Sertoli cells
Hum. Reprod., January 1, 2002; 17(1): 161 - 172.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
N. Cremades, M. Sousa, R. Bernabeu, and A. Barros
Developmental potential of elongating and elongated spermatids obtained after in-vitro maturation of isolated round spermatids
Hum. Reprod., September 1, 2001; 16(9): 1938 - 1944.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.