Molecular Human Reproduction, Vol. 6, No. 11, 1049-1053,
November 2000
© 2000 European Society of Human Reproduction and Embryology
Genetic diagnosis |
Study of DNA-methylation patterns at chromosome 15q11-q13 in children born after ICSI reveals no imprinting defects
1 Centre for Medical Genetics 2 Centre for Reproductive Medicine, University Hospital, Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Laarbeeklaan 101, B-1090 Brussels, Belgium
Abstract
The introduction of intracytoplasmic sperm injection (ICSI) has raised concern about safety in terms of a possible increase in the incidence of major congenital malformations, chromosomal aberrations or developmental problems. The possible influence of genetic imprinting on an ICSI procedure has not yet been investigated. We therefore studied the DNA-methylation status at a defined region in chromosome 15q11-q13 in 92 children born after an ICSI procedure. Imprinting defects in this region are associated with neurogenetic disorders, e.g. Angelman syndrome (AS) and Prader–Willi syndrome (PWS). Blood samples were taken directly after birth and stored at –80°C. Genomic DNA purification was performed from 3–7 ml EDTA–blood. Sodium bisulphite treatment was carried out in order to distinguish methylated from unmethylated DNA by transferring the unmethylated nucleic acid cytosine into uracil and leaving the methylated cytosine unchanged. Subsequently, a methylation-specific polymerase chain reaction (M-PCR) was performed. In all 92 children (83 from ICSI with ejaculated spermatozoa and nine from ICSI with non-ejaculated spermatozoa), a regular DNA-methylation pattern was found in the PWS/AS region. In none of the children were clinical symptoms of PWS or AS present. In conclusion, the results of this study do not indicate a higher risk of DNA-methylation defects in children born after ICSI.
children imprinting/DNA-methylation/ICSI/male infertility
Notes
3 To whom correspondence should be addressed at: Centre for Medical Genetics, University Hospital, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium. E-mail: lgenlsw{at}az.vub.ac.be
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M.V. Gomes, J. Huber, R.A. Ferriani, A.M. Amaral Neto, and E.S. Ramos Abnormal methylation at the KvDMR1 imprinting control region in clinically normal children conceived by assisted reproductive technologies Mol. Hum. Reprod., August 1, 2009; 15(8): 471 - 477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Seli and D. Sakkas Spermatozoal nuclear determinants of reproductive outcome: implications for ART Hum. Reprod. Update, July 1, 2005; 11(4): 337 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Lucifero, J.R. Chaillet, and J. M. Trasler Potential significance of genomic imprinting defects for reproduction and assisted reproductive technology Hum. Reprod. Update, January 1, 2004; 10(1): 3 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Devroey and A. Van Steirteghem A review of ten years experience of ICSI Hum. Reprod. Update, January 1, 2004; 10(1): 19 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Maher, M. Afnan, and C. L. Barratt Epigenetic risks related to assisted reproductive technologies: Epigenetics, imprinting, ART and icebergs? Hum. Reprod., December 1, 2003; 18(12): 2508 - 2511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Geuns, M. De Rycke, A. Van Steirteghem, and I. Liebaers Methylation imprints of the imprint control region of the SNRPN-gene in human gametes and preimplantation embryos Hum. Mol. Genet., November 15, 2003; 12(22): 2873 - 2879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Gianotten, F. van der Veen, M. Alders, N. J. Leschot, M. W.T. Tanck, J. A. Land, J. A.M. Kremer, L. H. Hoefsloot, M. M. Mannens, M. P. Lombardi, et al. Chromosomal region 11p15 is associated with male factor subfertility* Mol. Hum. Reprod., October 1, 2003; 9(10): 587 - 592. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Kurinczuk Safety issues in assisted reproduction technology: From theory to reality--just what are the data telling us about ICSI offspring health and future fertility and should we be concerned? Hum. Reprod., May 1, 2003; 18(5): 925 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. De Rycke, I. Liebaers, and A. Van Steirteghem Epigenetic risks related to assisted reproductive technologies: Risk analysis and epigenetic inheritance Hum. Reprod., October 1, 2002; 17(10): 2487 - 2494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ola, M. Afnan, K. Sharif, S. Papaioannou, N. Hammadieh, and C. L.R.Barratt Should ICSI be the treatment of choice for all cases of in-vitro conception?: Considerations of fertilization and embryo development, cost ffectiveness and safety Hum. Reprod., December 1, 2001; 16(12): 2485 - 2490. [Abstract] [Full Text] [PDF]
|
|