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Molecular Human Reproduction, Vol. 6, No. 12, 1165-1168, December 2000
© 2000 European Society of Human Reproduction and Embryology

Genetic diagnosis

Preimplantation genetic diagnosis for medium-chain acyl-CoA dehydrogenase (MCAD) deficiency

K. Sermon1,4, P. Henderix2, W. Lissens1, A.De Vos2, M. Vandervorst2, A. Vanderfaeillie1, E. Vamos3, A.Van Steirteghem2 and I. Liebaers1

1 Centre for Medical Genetics, and 2 Centre for Reproductive Medicine, University Hospital and Medical School of the Dutch-speaking Brussels Free University (Vrije Universiteit Brussel), Laarbeeklaan 101, 1090 Brussels, Belgium, and 3 Centre de Génétique, Université Libre de Bruxelles, Belgium

Abstract

Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is the most common defect in fatty acid oxidation. The disease is inherited in an autosomal recessive fashion (carrier frequency around 1 in 70) and probably affects as many as 1 in 10000 new-borns. Affected children usually present within the two first years of life with recurrent episodes of hypoketotic hypoglycaemia and lethargy leading to death in ~25% of the cases. One mutation (c985A->G) accounts for ~90% of the carrier chromosomes. We developed a preimplantation genetic diagnosis (PGD) strategy for MCAD for a couple who had already lost two affected children. When tested on heterozygous lymphoblasts, the amplification efficiency was 67 out of 71 (94%) and the allele drop-out rate was 0 out of 67. The patient became pregnant after one PGD cycle during which two embryos were replaced. The twin pregnancy was checked by chorionic villus sampling (CVS) and was shown to be unaffected. The twins have been born and are healthy.

medium-chain acyl-CoA dehydrogenase deficiency/preimplantation genetic diagnosis/single-cell PCR

Notes

4 To whom correspondence should be addressed. E-mail: lgensnk{at}az.vub.ac.be


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Home page
 J. Mol. Diagn.Home page
A. R. Thornhill and K. Snow
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J. Mol. Diagn., February 1, 2002; 4(1): 11 - 29.
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