Molecular Human Reproduction, Vol. 6, No. 2, 137-145,
February 2000
© 2000 European Society of Human Reproduction and Embryology
Ovary and oogenesis |
Expression of receptors for insulin-like growth factor-I and transforming growth factor-ß in human follicles
Laboratory of In Vitro Fertilization, Department of Gynecology, Hospital of Saint Luc, Catholic University of Louvain, Brussels, Belgium
Abstract
The in-vitro growth of immature oocytes in early follicles from cryopreserved human ovarian tissues is a new concept in in-vitro fertilization programmes for the treatment of infertile and cancer patients. To better understand the regulatory mechanism of follicular development, immunohistochemistry was used to study the expression of insulin-like growth factor (IGF) type I receptor (IGF-IR) and transforming growth factor-ß (TGFß) type I (TßR-I) and type II (TßR-II) receptors in fresh and frozen ovarian tissues from 14 women. Immunoreactivities for IGF-IR and TßR-I were present simultaneously in the oocytes of primordial, pre-antral and antral follicles. Staining for both IGF-IR and TßR-I was also observed in granulosa cells of primordial, pre-antral and antral follicles. IGF-IR and TßR-I also stained in thecal cells of pre-antral and antral follicles. Stromal cells in surrounding ovarian tissue expressed IGF-IR and TßR-I at various follicular stages. Unlike TßR-I, TßR-II was expressed only in the oocytes of primordial and primary follicles, and with weak staining intensity in thecal cells. No significant staining for TßR-II was found in oocytes and granulosa cells of antral follicles. There was no difference in staining patterns for IGF-IR, TßR-I and TßR-II between fresh and frozen ovarian tissues, indicating that cryopreservation might not significantly alter the immunoreactivities of these receptors in frozen ovarian tissue. The results suggest that IGF-I and TGFß may participate in the regulation of follicular growth by binding to their receptors through an autocrine or paracrine mechanism. IGF-I and TGFß may be useful in regulating the in-vitro or in-vivo maturation of oocytes not only in later follicles but also very early follicles, from cryopreserved ovarian tissues for clinical use in the future.
cryopreservation/follicle/insulin-like growth factor receptor/transforming growth factor-ß
Notes
1 To whom correspondence should be addressed at: Department of Gynaecology, Hospital of Saint Luc, Catholic University of Louvain (UCL), Avenue Hippocrate 10, UCL 10/9502, 1200 Brussels, Belgium
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