Molecular Human Reproduction, Vol. 6, No. 5, 397-403,
May 2000
© 2000 European Society of Human Reproduction and Embryology
Molecular endocrinology |
Evidence for nitric oxide acting as a luteolytic factor in the human corpus luteum
1 Department of Obstetrics and Gynecology, Göteborg University, Sahlgrenska University Hospital, 413 45 Göteborg, Sweden and 2 Department of Reproductive Medicine, University of California at San Diego, La Jolla, CA, USA
Abstract
The aims of the present study were to characterize the expression and cellular localization of isoforms of nitric oxide synthase (NOS) in the human corpus luteum (CL) and to determine the effects of nitric oxide (NO) on CL steroidogenesis. Immunoblotting analyses revealed that endothelial NOS (eNOS) is the most abundant isoform in human CL with highest values during the late luteal phase. Immunoreactive eNOS was localized predominantely in the theca lutein layer, being particularly abundant in endothelial cells, but with positive staining also in some steroidogenic cells. Immunoreactive inducible NOS (iNOS) was also detected, but to lesser degree, and did not display apparent phase-specific changes. The effect of NO on CL steroid synthesis was examined using human chorionic gonadotrophin (HCG)-stimulated dispersed CL cells cultured in vitro. Progesterone production was significantly decreased (P < 0.05) by the NO donor spermine NONOate (10–5 mol/l) in cells of the late, but not mid-, luteal phase. To investigate a potential link between NO and the local prostaglandins (PG), concentrations of PGF2
and PGE2 were measured in culture medium. NO significantly increased (P < 0.05) concentrations of both PGF2 and PGE2 during the late luteal phase. It is concluded that NO may be luteolytic in the human CL of menstruation.
corpus luteum/eNOS/NOS/nitric oxide/prostaglandins
Notes
3 To whom correspondence should be addressed
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