Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (4)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Sun, G.-H.
Right arrow Articles by Liu, H.-W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sun, G.-H.
Right arrow Articles by Liu, H.-W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Molecular Human Reproduction, Vol. 6, No. 5, 429-434, May 2000
© 2000 European Society of Human Reproduction and Embryology

Testis and spermatogenesis

Purification of GP-83, a glycoprotein secreted by the human epididymis and conjugated to mature spermatozoa

G.-H. Sun1,5, Y.-C. Lin2, Y.-W. Guo3, S.-Y. Chang1 and H.-W. Liu4

1 Division of Urology, Department of Surgery, Tri-Service General Hospital, National Defense Medical Center, Taipei, 2 Graduate Institute of Life Sciences, Department of Biology and Anatomy, National Defense Medical Center, Taipei, 3 Department of Biology and Anatomy, National Defense Medical Center, Taipei and 4 Graduate Institute of Medical Science, Department of Biology and Anatomy, National Defense Medical Center, Taipei, Taiwan 100, ROC

Abstract

Epididymal secretions are critical for mammalian spermatozoa to acquire both forward motility and an ability to recognize and penetrate oocytes. Previous studies identified two glycoproteins, GP-83 and GP-39, which were secreted by the human epididymis and may be related to maturation of sperm function. In this study, GP-83 was purified from human seminal fluid by DEAE–ion exchange, gel filtration chromatography and preparative gel elution. The isoelectric point (pI) of purified GP-83 was 6.57. Monospecific antiserum to GP-83 was induced in male New Zealand rabbits and confirmed on immunoblots. GP-83 was found in fluid, tissue and sperm extracts of corpus and cauda epididymis, but not in the caput. Immunohistochemical localization identified GP-83 in the luminal contents and in the supranuclear region and cell membrane of principal cells of the corpus and cauda epididymis. GP-83 was found on the anterior acrosome in ejaculated spermatozoa, and shifted to the equatorial region after capacitation and the acrosome reaction.

epididymis/glycoprotein/human/sperm maturation

Notes

5 To whom correspondence should be addressed


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
Y.-C. Lin, G.-H. Sun, Y.-M. Lee, Y.-W. Guo, and H.-W. Liu
Cloning and Characterization of a Complementary DNA Encoding a Human Epididymis-Associated Disintegrin and Metalloprotease 7 Protein
Biol Reprod, September 1, 2001; 65(3): 944 - 950.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.