Molecular Human Reproduction, Vol. 6, No. 6, 498-503,
June 2000
© 2000 European Society of Human Reproduction and Embryology
Ovary and oogenesis |
TGFß receptor types I and II and the substrate proteins Smad 2 and 3 are present in human oocytes
Reproductive Medical Center, Division of Obstetrics and Gynecology, Department of Women and Child Health, Karolinska Hospital, S-171 76 Stockholm, Sweden
Abstract
We have recently found that values of the transforming growth factor (TGF)ß1 in human ovarian follicular fluid obtained during ovarian stimulation for IVF were higher in women who subsequently became pregnant following embryo transfer. We therefore postulated that TGFß1 may have a beneficial effect on the preimplantation embryo and improve the chances of a successful implantation. We have used reverse transcription–polymerase chain reaction (RT–PCR) and immunohistochemistry to investigate the presence in human oocytes and preimplantation embryos of the essential components of the TGFß signalling pathway, TGFß receptors type I and II and the substrate proteins Smad 2 and 3. We found that both receptors, as well as Smad 2 and 3, were present in the unfertilized oocyte, whereas only the type I receptor and Smad 2 and 3 were present at the blastocyst stage. At the 4-cell and 8-cell stages neither of the receptors was present, but Smad 2 and 3 were present at both stages. These findings support our hypothesis that the TGFß1 in follicular fluid may interact with the oocyte and preimplantation embryo via TGFß receptors, and that TGFß signalling may be important for the development of the oocyte and the preimplantation embryo.
IVF/oocyte/signal transduction/Smad/TGFß
Notes
1 To whom correspondence should be addressed at: Division of Obstetrics and Gynecology, Reproductive Medical Center, Department of Woman and Child Health, Karolinska Hospital, S-171 76 Stockholm, Sweden. E-mail: gabriel.fried{at}fyfa.ki.se
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