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Molecular Human Reproduction, Vol. 6, No. 6, 504-509, June 2000
© 2000 European Society of Human Reproduction and Embryology

Ovary and oogenesis

Spontaneous luteinization of antral marmoset follicles in vitro

Uwe Wehrenberg1 and Gabriele M. Rune

Institute of Anatomy, Department of Cell Biology, E.M.A.-University, Friedrich Loeffler Strasse 23c, D-17487 Greifswald, Germany

Abstract

Large non-luteinized follicles of the marmoset monkey were cultured for up to 96 h in the presence of substances that are known to induce luteinization, i.e. LH, transforming growth factor (TGF)-ß and cyclic AMP. The state of the basal lamina, and the expression of connexin-43, {alpha}2 integrin subunit and TGF-ß receptor type II (TßR-II) were chosen as parameters to judge the progress of luteinization. Antral follicles, cultured for 1 h, were not luteinized, as shown by an intact basal lamina, strong immunoreactivity of connexin-43 in granulosa cells, and no expression of TßR-II in the theca layer. After 12 h, most follicles showed a dissolution of the basal lamina, a faint reactivity of connexin-43, high expression of TßR-II in theca- and outer granulosa cells and high expression of {alpha}2 integrin subunit in granulosa cells bordering at the basement membrane; all of which indicate luteinization. After 96 h of culture, luteal structures (e.g. corpora lutea accessoria) had developed. This was true for both non-stimulated and stimulated follicles. Our results strongly suggest that antral follicles luteinize spontaneously. The decisive determinant appears to be the follicular stage.

follicle culture/luteinization/ovary/primate/transforming growth factors

Notes

1 To whom correspondence should be addressed at: Institute of Anatomy, Department of Cell Biology, E.M.A.-University, Friedrich-Loeffler-Str. 23c, D-17487 Greifswald, Germany. E-mail wehrenbe{at}rz.uni-greifswald.de


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